Epidermoid metaplasias of xenotransplanted human tracheobronchial epithelium.
Epidermoid metaplasias of xenotransplanted human tracheobronchial epithelium.
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异种移植的人气管支气管上皮的表皮样化生。
DOI：
10.1093/carcin/7.6.987
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发表时间：
1986
期刊：
影响因子：
4.7
通讯作者：
Trump,BF
中科院分区：
文献类型：
--
作者：
Klein-Szanto,AJ;Baba,M;Trono,D;Obara,T;Resau,J;Trump,BF
通过将含有呼吸道粘膜的供体组织块或由Anin玻璃体扩增产生的上皮细胞导入去上皮化的大鼠气管中，实现了从中期尸检中获得的人气管支气管壁上皮细胞的再分化。在封闭气管，并将其移植到裸鼠的皮下组织后，新形成的上皮在裸露的管腔表面迁移。在这一过程中，观察到再生的表皮样化生，包括有角质化但没有异型性的薄层复层上皮的生长。异种移植4周后，管腔表面大部分被柱状上皮覆盖，偶见表皮样化生斑块。用7，12-二甲基苯并[a]菲(DMBA)处理该上皮细胞时，可观察到轻度至中度异型性的厚厚的表皮样化生。这种类型的上皮在将含有DMBA的小球插入气管管腔后一到三个月可见。抗角蛋白单抗AE1和AE3免疫组织化学染色显示，与未经处理的正常粘液纤毛上皮相比，再生化生组织和DMBA诱导的化生组织中的免疫染色均增强。虽然AE1和AE3在两种类型的化生中没有发现差异，但总蛋白免疫染色的使用显示了重要的差异。正常呼吸道上皮不含内化蛋白，但在再生的表皮样化生的表层可见内化蛋白。在DMBA诱导的化生组织中，不仅在浅层细胞中发现了包皮蛋白，而且在大量的超基细胞中也发现了它的存在。这些致癌物诱导的损伤的增生性，加上细胞异型性的存在和总蛋白分布模式的改变，提示为癌前状态。
Repopulation of rat tracheas of human tracheobronchial epithelial cells obtained from intermediate autopsies was achieved by introducing into de-epithelialized rat tracheas either pieces of donor tissue containing respiratory mucosa or epithelial cells produced by anin vitroamplification of these cells. After tracheas were sealed, and transplanted into the subcutaneous tissues of nude mice, a newly formed epithelium migrated over the denuded luminal surface. During this process, regenerative epidermoid metaplasias, consisting of the growth of thin stratified epithelium with keratinization but without atypia was observed. Four weeks after xenotransplantation, most of the luminal surface was covered by columnar epithelium with occasional patches of epidermoid metaplasia. When this epithelium was exposed to 7, 12-dimethylbenzo[a]anthracene (DMBA) a thick epidermoid metaplasia with mild to moderate atypia was observed. This type of epithelium is seen one to three months after insertion of the DMBA-containing pellets into the tracheal lumen. Immunohistochemical staining with antikeratin monoclonal antibodies AE1 and AE3 revealed increased immunostaining in both regenerative and DMBA-induced metaplasias compared with that of untreated normal mucociliary epithelium. Although no differences between the two types of metaplasias were detected with AE1 and AE3 the use of involucrin immunostain showed important differences. Normal respiratory epithelium did not contain in-volucrin, but this protein was seen in the surface layer of regenerative epidermoid metaplasias. In DMBA-induced metaplasias, involucrin was found not only in the superficial cells but was also present in numerous suprabasal cells. The hyperplastic nature of these carcinogen-induced lesions, together with the presence of cellular atypia and an altered involucrin distribution pattern, suggest a preneoplastic state.