Anti-infective mechanisms induced by a probiotic Lactobacillus strain against Salmonella enterica serovar Typhimurium infection
Anti-infective mechanisms induced by a probiotic Lactobacillus strain against Salmonella enterica serovar Typhimurium infection
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DOI：
10.1016/j.ijfoodmicro.2010.01.020
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发表时间：
2010-04-15
影响因子：
5.4
通讯作者：
Perdigon, Gabriela
中科院分区：
文献类型：
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作者：
de Moreno de LeBlanc, Alejandra;Castillo, Natalia A.;Perdigon, Gabriela
预防病原体感染是益生菌最广泛研究的效果之一。干酪乳杆菌CRL 431是一种益生菌，其对肠道免疫细胞的影响已被广泛研究。本研究的目的是利用小鼠模型，确定L。casei CRL 431以实现针对鼠伤寒沙门氏菌血清型感染的保护。在先前和连续（先前和感染后）益生菌给药中，分析了这种乳酸菌在肠道防御第一线（非特异性屏障和与肠道相关的先天免疫细胞）上诱导的机制，作为了解保护免受沙门氏菌鼠伤寒血清型的一些机制的一种方式。所获得的结果表明，在感染前7天施用Leasel CRL 431降低了鼠伤寒沙门氏菌血清变型感染的严重程度，表明连续施用（甚至在感染后）具有最佳效果。这种连续给药减少了肠道中病原体的计数及其在该器官外的传播。几种机制和细胞参与了这种对鼠伤寒沙门氏菌的保护作用。干酪乳杆菌CRL 431作用于细胞的先天性和适应性免疫应答。益生菌给药减少了嗜中性粒细胞浸润，从而减少了肠道炎症;激活了不同部位（如派尔集合淋巴结、脾脏和腹膜）的巨噬细胞吞噬活性;并增加了小肠固有层中伊加+细胞的数量，这与肠液中特异性抗病原体的s-IgA释放增加相关。对抑制细胞凋亡的机制尚不清楚。(c)2010 Elsevier B. V.保留所有权利。
The prevention of pathogen infections is one of the most extensively studied effects of probiotics. L casei CRL 431 is a probiotic bacterium and its effects on the gut immune cells have been extensively studied. The aim of the present study was to determine, using a mouse model, the preventive and therapeutic effect of L. casei CRL 431 to achieve protection against Salmonella enteritidis serovar Typhimurium infection. In both previous and continuous (previous and post-infection) probiotic administration, the mechanisms induced by this lactic acid bacteria on the first line of intestinal defense (non-specific barrier and the innate immune cells associated to the gut), as a way to understand some of the mechanisms involved in the protection against Salmonella enteritidis serovar Typhimurium, were analyzed. The results obtained demonstrated that 7 days L easel CRL 431 administration before infection decreased the severity of the infection with Salmonella enteritidis serovar Typhimurium, demonstrating that the continuous administration (even after infection) had the best effect. This continuous administration diminished the counts of the pathogen in the intestine as well as its spread outside this organ. Several mechanisms and cells are involved in this protective effect against Salmonella enteritidis serovar Typhimurium. L casei CRL 431 acted on cells of the innate and adaptive immune response. The probiotic administration decreased the neutrophil infiltration with the consequent diminution of intestinal inflammation; activated the macrophage phagocytic activity in different sites such as Peyer's patches, spleen and peritoneum; and increased the number of IgA + cells in the lamina propria of the small intestine which was correlated with increased release of s-IgA specific against the pathogen in the intestinal fluids. The mechanism of the inhibition of cellular apoptosis was not involved. (c) 2010 Elsevier B.V. All rights reserved.