The immunostimulating effect of bacterial genomic DNA on the innate immune responses of bivalve mussel, Hyriopsis cumingii Lea
The immunostimulating effect of bacterial genomic DNA on the innate immune responses of bivalve mussel, Hyriopsis cumingii Lea
复制标题
DOI：
10.1016/j.fsi.2005.12.013
复制
发表时间：
2006-10-01
影响因子：
4.7
通讯作者：
Shao, Jian-Zhong
中科院分区：
文献类型：
--
作者：
Hong, Xu-Tao;Xiang, Li-Xin;Shao, Jian-Zhong
利用含有未甲基化CpG基序的大肠杆菌基因组DNA，研究了细菌DNA对三角帆蚌（Hyriopsis cumingii）莱亚天然免疫应答的刺激作用。结果表明，E. coli DNA对血细胞体外产生超氧阴离子（O2-）和酸性磷酸酶（AP）无明显影响。然而，当细胞与50或100 μ g/ml细菌DNA孵育12和24小时时，血细胞的杀菌活性显著增加。当用50或100 μ g/ml的细菌DNA注射双壳贝类12和24 h时，血淋巴的抗菌活性、溶菌酶活性和酚氧化酶原（proPO）的产生也增加。48 h后这些活性恢复到对照水平。本研究表明，含有非甲基化CpG基序的细菌DNA在体内和体外均能激活双壳贝类免疫系统的某些参数。(c)2006爱思唯尔有限公司保留所有权利。
The genomic DNA of Escherichia coli, which contains the unmethylated CpG motif, was used to evaluate the immunostimulating effect of bacterial DNA on innate immune responses in the bivalve mussel Hyriopsis cumingii Lea. The results showed that the E. coli DNA had no significant effect on the production of superoxide anion (O2-) or acid phosphatase (AP) by haemocytes in vitro. However, the bactericidal activity of the haemocytes was significantly increased when the cells were incubated with 50 or 100 mu g/ml bacterial DNA for 12 and 24 h. Antibacterial activity, lysozyme activity, and prophenoloxidase (proPO) production of haemolymph were also increased, when the bivalve molluscs were injected with 50 or 100 mu g/ml of bacterial DNA for 12 and 24 h. These activities returned to the control level after 48 h. This work showed the bacterial DNA with unmethylated CpG motif could activate some parameters of the immune system of bivalve molluscs in vivo and in vitro. (c) 2006 Elsevier Ltd. All rights reserved.