Inhibitory antibodies against endopeptidase activity of human adamalysin 19.
Inhibitory antibodies against endopeptidase activity of human adamalysin 19.
复制标题
DOI：
10.1006/bbrc.2001.5958
复制
发表时间：
2001-11
影响因子：
3.1
通讯作者：
Yun-Ge Zhao;Ping Wei;Q. Sang
中科院分区：
文献类型：
--
作者：
Yun-Ge Zhao;Ping Wei;Q. Sang
人adamalysin 19（hADAM 19）/meltrin beta是ADAM（去整合素和金属蛋白酶）家族的成员，并且是活性金属蛋白酶。它是一种新的金属蛋白酶和去整合素树突状细胞抗原标志物。Adamalysin 19基因在正常和转化的组织和细胞如胎盘、脑、心脏、白细胞和结肠直肠腺癌SW 480中表达。为了开发特异性工具来研究hADAM 19的功能，合理地选择肽抗原并开发特异性多克隆抗体（pAb）来调节hADAM 19活性。抗金属蛋白酶和抗去整合素结构域IgG分子抑制hADAM 19对α-2-巨球蛋白的切割;然而，它们的免疫前和抗前结构域IgG分子却没有。由于抗去整合素IgG也中和蛋白水解活性，因此去整合素结构域可能影响hADAM 19蛋白折叠和/或底物结合。这些pAb可用于在组织和细胞中特异性定位hADAM 19蛋白，并阐明其生物学和病理学功能，如加工促生长因子。
Human adamalysin 19 (hADAM19)/meltrin beta is a member of the ADAM (a disintegrin and metalloproteinase) family and an active metalloproteinase. It is a new metalloproteinase and disintegrin dendritic cell antigen marker. Adamalysin 19 gene was expressed in normal and transformed tissues and cells such as placenta, brain, heart, leukocytes, and colorectal adenocarcinoma SW480. To develop specific tools to investigate the functions of hADAM19, peptide antigens were rationally selected and specific polyclonal antibodies (pAbs) were developed to modulate hADAM19 activity. Anti-metalloproteinase and anti-disintegrin domain IgG molecules inhibited the alpha-2-macroglobulin cleavage by hADAM19; however, their pre-immune and anti-pro-domain IgG molecules did not. Since anti-disintegrin IgG also neutralized the proteolytic activity, the disintegrin domain may affect the hADAM19 protein folding and/or substrate binding. These pAbs may be used to specifically localize the hADAM19 protein in tissues and cells and elucidate its biological and pathological functions such as processing pro-growth factors.