Annexin A8 is a novel molecular marker for detecting lymph node metastasis in oral squamous cell carcinoma.
Annexin A8 is a novel molecular marker for detecting lymph node metastasis in oral squamous cell carcinoma.
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DOI：
10.18632/oncotarget.6639
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发表时间：
2016-01-26
期刊：
影响因子：
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通讯作者：
Hamakawa H
中科院分区：
文献类型：
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作者：
Oka R;Nakashiro K;Goda H;Iwamoto K;Tokuzen N;Hamakawa H
颈部淋巴结转移是口腔鳞状细胞癌（OSCC）的重要预后因素，但前哨淋巴结活检或颈清扫术后的准确评估往往仅限于仅一两个切片的组织病理学检查。我们之前的研究表明，针对角蛋白19（KRT19）mRNA的逆转录环介导等温扩增（RT-LAMP）对于淋巴结转移的基因检测是有用的，但敏感性不足。在这里，我们试图鉴定淋巴结中 OSCC 细胞的新分子标记。我们进行了微阵列分析，以确定 OSCC 患者的 7 个转移性淋巴结中过表达的基因，与非癌症患者的 1 个正常淋巴结和 5 个唾液腺中的基因进行了比较。然后，我们使用实时定量 RT-PCR (qRT-PCR) 和 RT-LAMP 来比较这些基因在新切除的转移性淋巴结和正常淋巴结中的表达。在通过微阵列分析鉴定的 4 个基因中，通过 qRT-PCR 在转移性淋巴结中检测到膜联蛋白 A8 (ANXA8) 和桥粒芯糖蛋白 3 mRNA，但在正常淋巴结中未检测到。此外，在所有 KRT19 阴性转移淋巴结中均检测到 ANXA8 mRNA 表达。 KRT19 和 ANXA8 mRNA 可能是检测 OSCC 患者淋巴结转移的有用标记。
Cervical lymph node metastasis is an important prognostic factor in oral squamous cell carcinoma (OSCC), but its accurate assessment after sentinel node biopsy or neck dissection is often limited to the histopathological examination of only one or two sections. Previous our study showed the usefulness of the reverse transcription loop-mediated isothermal amplification (RT-LAMP) targeting keratin 19 (KRT19) mRNA for the genetic detection of lymph node metastasis, but the sensitivity was insufficient. Here, we have attempted to identify novel molecular markers for OSCC cells in lymph nodes. We performed microarray analysis to identify genes overexpressed in 7 metastatic lymph nodes from OSCC patients, compared to 1 normal lymph node and 5 salivary glands from non-cancer patients. We then used real-time quantitative RT-PCR (qRT-PCR) and RT-LAMP to compare the expression of these genes in newly resected metastatic and normal lymph nodes. Of 4 genes identified by microarray analysis, annexin A8 (ANXA8) and desmoglein 3 mRNA were detected by qRT-PCR in metastatic lymph nodes but not in normal lymph nodes. Furthermore, ANXA8 mRNA expression was detected in all KRT19-negative metastatic lymph nodes. Both KRT19 and ANXA8 mRNA may be useful markers for detecting lymph node metastases in OSCC patients.