Phosphatidylethanol in blood (B-PEth): A marker for alcohol use and abuse
Phosphatidylethanol in blood (B-PEth): A marker for alcohol use and abuse
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DOI：
10.1002/dta.278
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发表时间：
2011-04-01
影响因子：
2.9
通讯作者：
Alling, Christer
中科院分区：
文献类型：
--
作者：
Isaksson, Anders;Walther, Lisa;Alling, Christer
磷脂酰乙醇（PEth）代表一组甘油磷脂同系物，其中乙醇通过磷脂酶D结合在通常含有氨基醇的位置。由于PEth的形成特别依赖于乙醇，因此PEth作为酒精生物标志物的诊断特异性理论上为100%。PEth在血液中的半衰期约为4天。饮酒量与PEth的血液浓度相关，并且已显示PEth是比传统酒精标志物（例如CDT（碳水化合物缺乏型转铁蛋白）、GGT（γ-谷氨酰转移酶）和MCV（平均红细胞体积）或这些的组合）更敏感的饮酒指标。迄今为止，几乎所有可用的临床数据都是基于分析灵敏度有限的高效液相色谱（HPLC）方法。随着具有相当高的分析灵敏度的方法的出现（例如，G.质谱法），临床灵敏度将相应增加。然而，通过新的敏感分析技术测定极低浓度的PEth的可能性可能会产生必须考虑的伦理和法律的后果。版权所有（C）2011约翰威利父子有限公司
Phosphatidylethanol (PEth) represents a group of glycerophospholipid homologues where ethanol by phospholipase D has been bound at the position that normally contains an amino-alcohol. Since the formation of PEth is specifically dependent on ethanol, the diagnostic specificity of PEth as an alcohol biomarker is theoretically 100%. The half-life of PEth in blood is approximately 4 days. The amount of alcohol consumed correlates to blood concentration of PEth and PEth has been shown to be a more sensitive indicator of alcohol consumption than traditional alcohol markers, such as CDT (carbohydrate-deficient transferrin), GGT(gamma-glutamyl transferase), and MCV(mean corpuscular volume) or a combination of these. Almost all clinical data so far available are based on a high performance liquid chromatography (HPLC) method with limited analytical sensitivity. With the advent of methods with considerably higher analytical sensitivity (e. g. mass spectrometric methods), clinical sensitivity will increase correspondingly. The possibility of determining very low concentrations of PEth by new sensitive analytical techniques may, however, have both ethical and legal consequences that have to be considered. Copyright (C) 2011 John Wiley & Sons, Ltd.