Double innervation occurs in the facial mimetic muscles after facial-hypoglossal end-to-side neural repair: rat model for neural supercharge concept
Double innervation occurs in the facial mimetic muscles after facial-hypoglossal end-to-side neural repair: rat model for neural supercharge concept
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DOI：
10.1016/j.bjps.2007.01.075
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发表时间：
2008-01-01
影响因子：
2.7
通讯作者：
Yamamoto, Yuhei
中科院分区：
文献类型：
--
作者：
Furukawa, Hiroshi;Saito, Akira;Yamamoto, Yuhei
背景：面部双重神经支配。虽然面神经-舌下神经端侧缝合术已经用于持续性不完全性面神经，但是在端侧神经缝合术后面神经和舌下神经的模拟肌尚未得到证实。最近瘫痪，并已取得临床证据的恢复与罕见的联带运动。我们建立了一个大鼠模型，比较了面神经和舌下神经端端缝合和端侧缝合后的联带运动，并通过逆行示踪证实了双神经支配。方法：大鼠分为三组（每组6只）、面神经麻痹组（A组）、面-舌下神经端端吻合组（B组）、面神经-舌下神经端侧吻合组（C组）。手术后八周，面部的联动。通过摄像机观察并记录模拟肌。C组：术后向面神经模拟肌内注射神经示踪剂（Fast Blue、Diamidino Yellow和Dil），以证实面神经和舌下神经的双重支配。然而，白色进食和饮水时，他们的半边脸出现了集体运动（胡须垫肌肉强烈收缩，鼻子弯曲和眼睛闭合）。在C组中，4只大鼠未显示显著变化，然而，2只大鼠显示眼睑白色进食和饮水的联带运动（与对侧可区分的频繁闭眼）。正常侧）。C组大鼠面神经和舌下神经运动核均原位检测到逆行示踪剂注入模拟肌。结论：神经端侧吻合后，面神经和舌下神经对模拟肌有双重神经支配。舌下神经-面神经端侧吻合后口周双神经支配的模拟肌的联带运动较端端吻合少。(c)2007年英国整形、重建和美容外科医生协会。由爱思唯尔有限公司出版。保留所有权利。
Background: Double innervation of facial. mimetic muscles by both facial and hypoglossal nerves after end-to-side neurorrhaphy has not been proven, although facial -hypoglossal end-to-side neurorrhaphy has been used in persistent incomplete facial. palsy recently, and has achieved clinical evidences of recovery with rare synkinesis. We established a rat model to compare synkinesis after end-to-end and end-to-side neurorrhaphy techniques between facial and hypoglossal nerves, and confirmed double innervation using retrograde tracers.Methods: Rats were divided into three groups (each consisting of six rats), a facial palsy group (Group A), a facial-hypoglossal end-to-end neurorrhaphy group (Group B), and a facial-hypoglossal end-to-side neurorrhaphy group (Group C). Eight weeks after surgery, synkinesis of the facial. mimetic muscles was observed and recorded via video camera. In Group C, post operative, intramuscular injections of retrograde neural tracers (Fast Blue, Diamidino Yellow and Dil) into the facial mimetic muscles were performed to prove double innnervation by both the facial and hypoglossal nerves.Results: In Group B, all rats showed facial, palsy. However white eating and drinking, their half of the face showed mass movements (strong contraction of whisker pad muscles, curved nose and eye-closure). In Group C, four rats showed no significant changes however, two rats showed synkinesis of the eyelid white eating and drinking (frequent eye-closure distinguishable from the contralateral. normal side). In Group C, retrograde tracers injected in the mimetic muscles were detected in both the facial and hypoglossal motor nuclei in situ of all the rats' brain stem.Conclusion: This study proved that double innervation of mimetic muscles by both facial and hypoglossal nerves occurs after the end-to-side neurorrhaphy. Double-innervated mimetic muscles around the mouth after hypoglossal-facial end-to-side neurorrhaphy showed Less synkinesis than the end-to-end neurorrhaphy. (c) 2007 British Association of Plastic, Reconstructive and Aesthetic Surgeons. Published by Elsevier Ltd. All rights reserved.