smyd1 and smyd2 are expressed in muscle tissue in Xenopus laevis
smyd1 and smyd2 are expressed in muscle tissue in Xenopus laevis
复制标题
DOI：
10.1007/s10616-008-9128-1
复制
发表时间：
2008-06-01
期刊：
影响因子：
2.2
通讯作者：
Tashiro, Kosuke
中科院分区：
文献类型：
--
作者：
Kawamura, Shinobu;Yoshigai, Emi;Tashiro, Kosuke
组蛋白的表观遗传修饰在细胞分裂、细胞死亡和细胞分化等细胞活动的调控中起着重要作用。由约130个氨基酸组成的SET结构域在共底物S-腺苷甲硫氨酸存在下具有赖氨酸甲基转移酶活性。在各种生物体中已经预测了超过60种含有SET结构域的蛋白质。其中，SMYD家族基因含有SET结构域和锌指MYND结构域，据报道其调节细胞周期和肌肉形成。在这里，我们研究的表达和功能的smart 1和2在非洲爪蟾。Smad 1和Smad 2在各种肌肉组织中表达。Smad 3 1主要在心肌和骨骼肌中表达，Smad 3 2在骨骼肌和面部表达丰富。此外，通过使用反义morpholino寡核苷酸的功能丧失实验，表明smart 1和2与肌细胞分化有关。
Epigenetic modifications of histone play important roles for regulation of cell activity, such as cell division, cell death, and cell differentiation. A SET domain consisting of about 130 amino acids has lysine methyltransferase activity in the presence of the cosubstrate S-adenosyl-methionine. More than 60 SET domain-containing proteins have been predicted in various organisms. One of them, the SMYD family genes which contain a SET domain and a zinc-finger MYND domain are reported to regulate cell cycle and muscle formation. Here we examined the expression and function of smyd1 and 2 in Xenopus. smyd1 and 2 were expressed in various muscle tissues. While smyd1 expression was observed mainly in cardiac muscle and skeletal muscle, smyd2 expression was done abundantly in skeletal muscle and face region. Moreover, by loss-of-function experiments using antisense morpholino oligonucleotides, it was suggested that smyd1 and 2 related to muscle cells differentiation.