Mechanical Heterogeneity in the Bone Microenvironment as Characterized by Atomic Force Microscopy
Mechanical Heterogeneity in the Bone Microenvironment as Characterized by Atomic Force Microscopy
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DOI：
10.1016/j.bpj.2020.06.026
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发表时间：
2020-08-04
影响因子：
3.4
通讯作者：
Hobbs, Jamie K.
中科院分区：
文献类型：
--
作者：
Chen, Xinyue;Hughes, Russell;Hobbs, Jamie K.
骨骼是具有多种功能的结构异质器官，在多种长度尺度上承受机械刺激。骨微环境的力学表征对于理解骨骼在健康和疾病中的功能是很重要的。在这里，我们描述了新鲜小鼠骨骼中的皮质骨、生长板、干骺端和骨髓的力学结构，并在生理缓冲液中使用原子力显微镜进行了探测。弹性和粘弹性性质都是高度不均匀的，模量范围在三到五个数量级之间，无论是在区域内还是在区域之间。所有区域都包括非常柔韧的区域，模量为几帕斯卡，黏度低至几十Pa中心点s。老化对骨髓的粘弹性有强烈的影响，但对其他研究区域的影响有限。我们的方法为探索与细胞过程相关的复杂组织的长度尺度的机械特性以及这些力学特性如何影响衰老和疾病提供了机会。
Bones are structurally heterogeneous organs with diverse functions that undergo mechanical stimuli across multiple length scales. Mechanical characterization of the bone microenvironment is important for understanding how bones function in health and disease. Here, we describe the mechanical architecture of cortical bone, the growth plate, metaphysis, and marrow in fresh murine bones, probed using atomic force microscopy in physiological buffer. Both elastic and viscoelastic properties are found to be highly heterogeneous with moduli ranging over three to five orders of magnitude, both within and across regions. All regions include extremely compliant areas, with moduli of a few pascal and viscosities as low as tens of Pa center dot s. Aging impacts the viscoelasticity of the bone marrow strongly but has a limited effect on the other regions studied. Our approach provides the opportunity to explore the mechanical properties of complex tissues at the length scale relevant to cellular processes and how these impact aging and disease.