Hyperinfectivity of human-passaged Vibrio cholerae can be modeled by growth in the infant mouse
Hyperinfectivity of human-passaged Vibrio cholerae can be modeled by growth in the infant mouse
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DOI：
10.1128/iai.73.10.6674-6679.2005
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发表时间：
2005-10-01
影响因子：
3.1
通讯作者：
Calderwood, SB
中科院分区：
文献类型：
--
作者：
Alam, A;LaRocque, RC;Calderwood, SB
先前已经表明，霍乱弧菌通过人类肠道的通道赋予可能导致霍乱流行性传播的瞬时高感染性表型。这种人类传代的高感染性的机制尚不完全清楚，部分原因是回收和研究人类粪便中新鲜通过的生物体存在固有的困难。在这里，我们表明，通过幼鼠肠道的V霍乱导致同等程度的高感染性通过人类宿主。我们已经使用了这个婴儿小鼠模型的主机传代的高感染性的时间和解剖位置的竞争优势，小鼠传代V胆固醇，以及三个IV型皮利的表型的贡献。
It has previously been shown that passage of Vibrio cholerae through the human intestine imparts a transient hyperinfectious phenotype that may contribute to the epidemic spread of cholera. The mechanism underlying this human-passaged hyperinfectivity is incompletely understood, in part due to inherent difficulties in recovering and studying organisms that are freshly passed in human stool. Here, we demonstrate that passage of V cholerae through the infant mouse intestine leads to an equivalent degree of hyperinfectivity as passage through the human host. We have used this infant mouse model of host-passaged hyperinfectivity to characterize the timing and the anatomic location of the competitive advantage of mouse-passaged V cholerae as well as the contribution of three type IV pili to the phenotype.