MUTATIONS AFFECTING STRUCTURE OF HYPOXANTHINE-GUANINE PHOSPHORIBOSYLTRANSFERASE IN CULTURED CHINESE-HAMSTER CELLS
MUTATIONS AFFECTING STRUCTURE OF HYPOXANTHINE-GUANINE PHOSPHORIBOSYLTRANSFERASE IN CULTURED CHINESE-HAMSTER CELLS
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DOI：
10.1073/pnas.70.2.320
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发表时间：
1973-01-01
影响因子：
11.1
通讯作者：
CASKEY, CT
中科院分区：
文献类型：
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作者：
BEAUDET, AL;ROUFA, DJ;CASKEY, CT
从经诱变处理的中国仓鼠培养细胞中分离的14个8-氮鸟嘌呤抗性克隆，检测了次黄嘌呤：鸟嘌呤磷酸核糖基转移酶的酶活性和免疫活性的丧失。三个克隆没有酶活性，但强烈的反应与抗血清制备的酶（CRM+），表明存在缺陷的酶蛋白，可能是由其结构基因的突变引起的。其他11个突变体几乎没有或没有酶或免疫活性。具有阳性酶和交叉反应免疫活性的回复突变体克隆来自这两类突变体。该抗血清已用于免疫吸附亲和层析法快速纯化中国仓鼠细胞中的磷酸核糖基转移酶。
14 Clones resistant to 8-azaguanine, isolated from mutagenically treated cultured cells from Chinese hamsters, were tested for loss of enzymic and immunological activities of hypoxanthine: guanine phosphoribosyltransferase. Three of the clones had no enzymic activity but reacted strongly with antiserum prepared against the enzyme (CRM+), indicating the presence of a defective enzyme protein, probably caused by a mutation in its structural gene. The other 11 mutants had little or no enzymic or immunologic activity. Revertant clones with positive enzymic and crossreacting immunologic activities were derived from both classes of mutants. The antiserum has been used for rapid purification of the phosphoribosyltransferase from Chinese hamster cells by immunoadsorbant affinity chromatography.