The need for IgG2c specific antiserum when isotyping antibodies from C57BL/6 and NOD mice
The need for IgG2c specific antiserum when isotyping antibodies from C57BL/6 and NOD mice
复制标题
DOI：
10.1016/s0022-1759(98)00015-5
复制
发表时间：
1998-03-15
影响因子：
2.2
通讯作者：
Lew, AM
中科院分区：
文献类型：
--
作者：
Martin, RM;Brady, JL;Lew, AM
小鼠IgG2a抗体的同型和定量广泛应用于针对BALB/c IgG2a骨髓瘤蛋白的商业单克隆和多克隆抗血清。最近发现，具有Ighl-b等位基因的近交系小鼠不具有IgG2a基因，而是表达IgG2c同型。我们发现，在免疫印迹和ELISA中，商业抗igg2a血清与IgG2c的交叉反应不充分，因此不适合在C57BL/6、C57BL/10和NOD等小鼠品系中检测和测量这一亚类。我们已经使用DNA免疫来产生多克隆抗igg2c血清，并证明使用igg2c特异性抗血清来准确量化具有Ighl-b等位基因的小鼠菌株的同型反应是必要的。(C) 1998 Elsevier Science B.V.版权所有
Isotyping and quantitation of murine IgG2a antibodies are widely performed with commercial monoclonal and polyclonal antisera raised against BALB/c IgG2a myeloma proteins. Recently it became evident that inbred mouse strains with the Ighl-b allele do not have the gene for IgG2a and instead express the IgG2c isotype. We show that commercial anti-IgG2a sera cross-react inadequately against IgG2c in immunoblot and ELISA and hence, are not suitable to detect and measure this subclass in mouse strains such as C57BL/6, C57BL/10 and NOD. We have used DNA immunization to generate polyclonal anti-IgG2c serum and demonstrated that it is essential to use IgG2c-specific antiserum to quantify accurately isotypic responses in mouse strains with the Ighl-b allele. (C) 1998 Elsevier Science B.V. All rights reserved.