The Arabidopsis thaliana SOS2 gene encodes a protein kinase that is required for salt tolerance.
The Arabidopsis thaliana SOS2 gene encodes a protein kinase that is required for salt tolerance.
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DOI：
10.1073/pnas.97.7.3730
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发表时间：
2000-03
影响因子：
11.1
通讯作者：
Jiping Liu;M. Ishitani;U. Halfter;Cheol-Soo Kim;Jian‐Kang Zhu
中科院分区：
文献类型：
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作者：
Jiping Liu;M. Ishitani;U. Halfter;Cheol-Soo Kim;Jian‐Kang Zhu
在拟南芥中，盐过度敏感2（SOS 2）基因是细胞内Na（+）和K（+）稳态所必需的。SOS 2基因突变导致Na（+）和K（+）失衡，使植物对高Na（+）和低K（+）环境的生长抑制更敏感。我们通过定位克隆的方法分离了SOS 2基因。预测SOS 2编码丝氨酸/苏氨酸型蛋白激酶，其N-末端催化结构域类似于酵母SNF 1激酶。对SOS 2突变等位基因的序列分析表明，SOS 2的N-末端催化结构域和C-末端调节结构域在功能上是必需的。根中SOS 2转录的稳态水平被盐胁迫上调。自磷酸化分析表明，SOS 2是一种活性蛋白激酶。在隐性sos 2 -5等位基因中，激酶催化结构域中保守的甘氨酸残基被改变为谷氨酸。该突变消除了SOS 2自磷酸化，表明SOS 2蛋白激酶活性是耐盐性所必需的。
In Arabidopsis thaliana, the Salt Overly Sensitive 2 (SOS2) gene is required for intracellular Na(+) and K(+) homeostasis. Mutations in SOS2 cause Na(+) and K(+) imbalance and render plants more sensitive toward growth inhibition by high Na(+) and low K(+) environments. We isolated the SOS2 gene through positional cloning. SOS2 is predicted to encode a serine/threonine type protein kinase with an N-terminal catalytic domain similar to that of the yeast SNF1 kinase. Sequence analyses of sos2 mutant alleles reveal that both the N-terminal catalytic domain and the C-terminal regulatory domain of SOS2 are functionally essential. The steady-state level of SOS2 transcript is up-regulated by salt stress in the root. Autophosphorylation assays show that SOS2 is an active protein kinase. In the recessive sos2-5 allele, a conserved glycine residue in the kinase catalytic domain is changed to glutamate. This mutation abolishes SOS2 autophosphorylation, indicating that SOS2 protein kinase activity is required for salt tolerance.