Isolation and characterization of Deinococcus geothermalis T27, a slightly thermophilic and organic solvent-tolerant bacterium able to survive in the presence of high concentrations of ethyl acetate
Isolation and characterization of Deinococcus geothermalis T27, a slightly thermophilic and organic solvent-tolerant bacterium able to survive in the presence of high concentrations of ethyl acetate
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DOI：
10.1111/j.1574-6968.2008.01273.x
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发表时间：
2008-09-01
影响因子：
2.1
通讯作者：
Vangnai, Alisa S.
中科院分区：
文献类型：
--
作者：
Kongpol, Ajiraporn;Kato, Junichi;Vangnai, Alisa S.
在45 ℃下，在作为唯一碳源提供的甲苯蒸气的存在下，分离出耐溶剂的轻微嗜热细菌。菌株T27经鉴定为地热异常球菌（Deinococcus geothermalis）T27。它可以耐受作为非水层（5%和20%，v/v）提供给细胞悬浮液的高浓度溶剂，并且具有显著的耐受log P（ow）值范围从正癸烷的5.6到低至乙酸乙酯的0.7的宽范围溶剂的能力。它还能够在45摄氏度下利用一些测试溶剂作为生长基质。Ca ~（2+）、葡萄糖和果糖的加入部分促进了耐溶剂性。暴露于乙酸乙酯的细胞似乎具有较小的尺寸;然而，即使在溶剂休克后，细胞结构也没有改变，并且明显清晰。D. geothermalis T27在高水平的有毒溶剂胁迫存在下在相对较高的温度下的降解表明其在生物技术应用以及外源性物质的生物修复中的潜在用途。
A solvent-tolerant, slightly thermophilic bacterium was isolated at 45 degrees C in the presence of toluene vapor provided as the sole carbon source. Strain T27 was identified as Deinococcus geothermalis T27. It could tolerate high concentrations of solvent provided as a nonaqueous layer (5% and 20%, v/v) to a cell suspension and had a remarkable ability to tolerate a broad range of solvents having log P(ow) values ranging from 5.6 of n-decane to as low as 0.7 of ethyl acetate. It was also able to utilize some of the solvents tested as a growth substrate at 45 degrees C. The addition of Ca(2+) ion, glucose and fructose partially promoted solvent tolerance. Cells exposed to ethyl acetate appeared to have a smaller size; however, the cell structure was not altered and was apparently well defined even after solvent shock. The tolerance of D. geothermalis T27 in the presence of high levels of toxic solvent stress at a comparatively high temperature indicated its potential use in biotechnological applications as well as bioremediation of xenobiotics.