Comparative study of the cell tropism of feline immunodeficiency virus isolates of subtypes A, B and D classified on the basis of the env gene V3-V5 sequence
Comparative study of the cell tropism of feline immunodeficiency virus isolates of subtypes A, B and D classified on the basis of the env gene V3-V5 sequence
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DOI：
10.1099/0022-1317-77-1-93
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发表时间：
1996-01-01
影响因子：
3.8
通讯作者：
Koyama, H
中科院分区：
文献类型：
--
作者：
Hohdatsu, T;Hirabayashi, H;Koyama, H
猫免疫缺陷病毒（FIV）分离物根据环境基因V3-V5序列可分为A、B、C和D亚型。比较了7株日本新分离株和1株Petaluma（原型）FIV亚型（a、B、D）对猫科淋巴母细胞样细胞株和猫科成纤维母细胞样细胞株的细胞趋向性。以FeT-1 （CD4（+/-）、CD8（-）和CD9(++)）细胞和Kumi-1 （CD4（++）、CD8（-）和CD9(++)）细胞作为IL-2依赖性猫t淋巴细胞系，以FeT-J (CD4（+）、CD8（+/-）和CD9（++）细胞和3201 (CD4（++）、CD8（+）和CD9（-）细胞作为IL-2依赖性猫t淋巴细胞系。使用的猫成纤维样细胞系为克兰德尔猫肾（CrFK）和fcwf-4 (CD4（-), CD8（-）和CD9(++)）细胞。所有FIV分离株在所有使用的淋巴母细胞细胞系中复制。所有分离株对Kumi-1细胞均表现出最强的细胞致病性。所有分离株即使在cd9阴性的3201细胞中也能复制。与il -2依赖性细胞系相比，il -2非依赖性细胞系中更多的分离株引起持续感染。建立持续感染的B亚型分离株数量有限，4株中只有1株。只有A亚型分离物u在CrFK细胞中复制，而没有分离物在具有与CrFK细胞相似的细胞表面标记的fcwf-4细胞中复制。在CrFK细胞中生长的A亚型病毒（CrFK/Petaluma、CrFK/Sendai-1）对淋巴母细胞的致病性高于在淋巴母细胞中生长的A亚型病毒（FL-4/Petaluma、Kumi-1/Sendai-1）。
Feline immunodeficiency virus (FIV) isolates have been classified into subtypes A, B, C and D based on the env gene V3-V5 sequence. The cell tropism of seven new Japanese isolates and a Petaluma (prototype) isolate of FIV, which classified into subtypes A, B and D, for feline lymphoblastoid and feline fibroblastoid cell lines was compared. FeT-1 (CD4(+/-), CD8(-) and CD9(++)) and Kumi-1 (CD4(++), CD8(-) and CD9(++)) cells were used as the interleukin-2 (IL-2)-dependent feline T-lymphocyte cell lines and FeT-J (CD4(+), CD8(+/-) and CD9(++)) and 3201 (CD4(++), CD8(+) and CD9(-)) cells were used as the IL-2-independent feline T-lymphocyte cell lines. The feline fibroblastoid cell lines used were Crandell feline kidney (CrFK) and fcwf-4 (both CD4(-), CD8(-) and CD9(++)) cells. All FIV isolates replicated in all lymphoblastoid cell lines used. All isolates showed the greatest cytopathogenicity for Kumi-1 cells. All isolates replicated even in the CD9-negative 3201 cells. More isolates caused persistent infection in IL-2-independent cell lines than in IL-2-dependent cell lines. The number of subtype B isolates that established persistent infection was limited, only one of four strains. Only the subtype A isolates u replicated in CrFK cells, whereas none of the isolates replicated in fcwf-4 cells, which have similar cell surface markers to CrFK cells. The subtype A viruses (CrFK/Petaluma, CrFK/Sendai-1) growing in CrFK cells showed greater cytopathogenicity for lymphoblastoid cell lines than did those (FL-4/Petaluma, Kumi-1/Sendai-1) growing in a lymphoblastoid cell line.