ENZYME-LINKED IMMUNOSORBENT-ASSAY FOR THE DETECTION OF THE CORONAVIRUS-LIKE AGENT AND ITS ANTIBODIES IN PIGS WITH PORCINE EPIDEMIC DIARRHEA
ENZYME-LINKED IMMUNOSORBENT-ASSAY FOR THE DETECTION OF THE CORONAVIRUS-LIKE AGENT AND ITS ANTIBODIES IN PIGS WITH PORCINE EPIDEMIC DIARRHEA
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DOI：
10.1016/0378-1135(82)90009-8
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发表时间：
1982-01-01
影响因子：
3.3
通讯作者：
PENSAERT, M
中科院分区：
文献类型：
--
作者：
CALLEBAUT, P;DEBOUCK, P;PENSAERT, M
建立了一种检测猪流行性腹泻（PED）自然感染和CV777分离株实验感染猪粪便中冠状病毒样因子的酶联免疫吸附试验（ELISA）。该方法特异性强，灵敏度高于EM。描述了用于检测和滴定抗体的ELISA阻断测定。在实验感染CV777的猪和自然感染PED的猪中证实了特异性抗体形成。
An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of the coronavirus-like agent in feces of pigs naturally affected with porcine epidemic diarrhea (PED) or experimentally infected with the CV777 isolate. The assay was specific and more sensitive than EM. An ELISA blocking assay is described for the detection and titration of antibodies. Specific antibody formation was demonstrated in pigs experimentally infected with CV777 and in swine naturally affected with PED.