Surface-linked liposomal antigen induces IgE-selective unresponsiveness regardless of the lipid components of liposomes
Surface-linked liposomal antigen induces IgE-selective unresponsiveness regardless of the lipid components of liposomes
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DOI：
10.1021/bc0001185
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发表时间：
2001-05-01
影响因子：
4.7
通讯作者：
Uchida, T
中科院分区：
文献类型：
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作者：
Nakano, Y;Mori, M;Uchida, T
我们以前曾报道，抗原与脂质体偶联诱导小鼠抗原特异性和IgE选择性无反应性。研究了该抗原制剂在新型疫苗方案中的应用，以诱导最小的IgE合成。在这项研究中，卵清蛋白（OVA）-脂质体缀合物使用四种不同的脂质体组分，包括不饱和载体脂质和三种不同的饱和载体脂质，在此之后，在小鼠中研究了诱导抗-OVA抗体的产生。所有的OVA-脂质体缀合物诱导IgE选择性无反应性。脂质体的膜流动性，通过检测位于双层中的1，6-二苯基-1，3，5-己三烯（DPH）探针的荧光偏振的变化来测量，在由不饱和载体脂质组成的脂质体中显著高于由饱和载体脂质组成的其它脂质体。在用使用由不饱和载体脂质组成的脂质体制备的OVA-脂质体免疫的小鼠中观察到抗OVA IgG的最高滴度。此外，在这些OVA-脂质体中，具有最长碳链的脂质体诱导最低的IgG抗体产生。这些结果表明，脂质体的膜流动性可能会影响脂质体的佐剂效应，但不与表面连接的脂质体抗原免疫的IgE选择性无反应性的诱导。
We have previously reported that antigen coupled with liposomes induced antigen-specific and IgE-selective unresponsiveness in mice. This antigen preparation was investigated for application in a novel vaccine protocol to induce minimal IgE synthesis. In this study, ovalbumin (OVA)-liposome conjugates were made using liposomes of four different lipid components, including unsaturated carrier lipid and three different saturated carrier lipids, after which the induction of anti-OVA antibody production was investigated in mice. All of the OVA-liposome conjugates induced IgE-selective unresponsiveness. The membrane fluidity of liposomes, as measured by detecting changes in the fluorescence polarization of a 1,6-diphenyl-1,3,5-hexatriene (DPH) probe located in the bilayers, was significantly higher in liposomes consisting of unsaturated carrier lipids than those of the other liposomes consisting of saturated carrier lipids. The highest titer of anti-OVA IgG was observed in mice immunized with OVA-liposomes made using liposomes consisting of unsaturated carrier lipids. In addition, among these OVA-liposomes, the one possessing the longest carbon chain induced the lowest IgG antibody production. These results suggest that the membrane fluidity of liposomes might affect the adjuvant effect of liposomes but not the induction of IgE-selective unresponsiveness in immunizations with surface-linked liposomal antigens.