Asymmetric somatic hybrids between Lycopersicon esculentum and irradiated Lycopersicon peruvianum
Asymmetric somatic hybrids between Lycopersicon esculentum and irradiated Lycopersicon peruvianum
复制标题
番茄和辐照秘鲁番茄之间的不对称体细胞杂交
DOI：
10.1007/bf00210064
复制
发表时间：
1990
影响因子：
5.4
通讯作者：
M. Koornneef
中科院分区：
文献类型：
--
作者：
J. Wijbrandi;A. Posthuma;J. M. Kok;R. Rijken;J. M. Vos;M. Koornneef
Asymmetric somatic hybrids ofLycopersicon esculentumandLycopersicon peruvianumwere obtained by fusion of leaf protoplasts from both species after irradiation of protoplasts or leaf tissue ofL. peruvianumwith 50, 300, or 1,000 Gy of gamma-rays. These radiation doses were sufficient to abolish the growth of theL. peruvianumprotoplasts. The hybrids were selected for their ability to regenerate plants; this regeneration capacity derived fromL. peruvianum. All asymmetric hybrid plants were aneuploid. The ploidy level, the morphology, and the regeneration rate were analyzed in relation to the radiation dose applied toL. peruvianum. After a low dose (50 Gy), most hybrids had near-triploid chromosome numbers, whereas after a high dose (300 or 1,000 Gy), most hybrids had near-pentaploid numbers. The morphology of the asymmetric hybrids was intermediate between that ofL. esculentumand symmetric somatic hybrids of both species (obtained without irradiation treatment), and approached the morphology ofL. esculentumto a greater extent after a high dose of irradiation. The asymmetric hybrids regenerated more slowly than the symmetric hybrids and regeneration proceeded more slowly after a high dose than after a low dose of irradiation. The high-dose hybrids also grew more slowly, flowered less, and set fruits less than the low-dose hybrids. No seeds could be obtained from any asymmetric hybrid.
