Characterization and expression of embryonic and adult globins of the teleost Oryzias latipes (Medaka)
Characterization and expression of embryonic and adult globins of the teleost Oryzias latipes (Medaka)
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DOI：
10.1093/oxfordjournals.jbchem.a003260
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发表时间：
2002-10-01
影响因子：
2.7
通讯作者：
Iuchi, I
中科院分区：
文献类型：
--
作者：
Maruyama, K;Yasumasu, S;Iuchi, I
使用硬骨鱼 Oryzias latipes (medaka)，我们从受精后 5 天（30℃）的胚胎中分离出 3 个胚胎珠蛋白 cDNA（em.alpha-0、em.alpha-1 和 em.beta-1），从完全生长的成鱼肾脏中分离出 2 个成体球蛋白 cDNA（ad.alpha-1 和 ad.beta-1），并预测了它们的氨基酸序列。分子系统发育分析表明，胚胎珠蛋白的氨基酸序列与先前在虹鳟鱼和斑马鱼中鉴定的胚胎珠蛋白高度同源，并且它们在硬骨鱼珠蛋白分子中形成单系群。它们与成年青鳉珠蛋白明显不同。 RT-PCR分析表明，胚胎珠蛋白mRNA在30和38期胚胎以及孵化后30天的幼鱼中强烈表达。幼鱼阶段后表达水平急剧下降，并且在完全生长的成鱼中表达水平较低。成体α珠蛋白mRNA ad.alpha-1在胚胎中几乎不表达，而在幼鱼至完全长大的成鱼中表达水平逐渐增加。出乎意料的是，成体β珠蛋白mRNA ad.beta-1在整个生命周期中表达，从早期胚胎阶段到完全生长的成体阶段。这种表达谱与之前研究的虹鳟鱼的表达谱有很大不同。青鳉的一些球蛋白在原始造血和最终造血中都有表达。
Using the teleost Oryzias latipes (medaka), we isolated three embryonic globin cDNAs (em.alpha-0, em.alpha-1, and em.beta-1) from the embryos 5 days after fertilization (at 30degreesC) and two adult globin cDNAs (ad.alpha-1 and ad.beta-1) from the kidney of the fully-grown adult fish, and predicted their amino acid sequences. Molecular phylogenetic analysis showed that the embryonic globins were highly homologous in amino acid sequence to the embryonic globins previously identified in rainbow trout and zebrafish, and that they formed a monophyletic group among the teleostean globin molecules. They were clearly discriminated from the adult globin of the medaka. RT-PCR analysis showed that the embryonic globin mRNAs were intensely expressed in stage 30 and 38 embryos and in young fish 30 days after hatching. The level of expression decreased drastically after the young fish stage, and was low in fully-grown adult fish. The adult alpha globin mRNA ad.alpha-1 was scarcely expressed in the embryos, and the level of expression gradually increased in young to fully-grown adult fish. Unexpectedly, the adult beta globin mRNA ad.beta-1 was expressed throughout life, from the early embryonic stage to the fully-grown adult stage. This expression profile was quite different from that of the rainbow trout previously investigated. Some globins of the medaka were expressed both in primitive hematopoiesis and in definitive hematopoiesis.