Ground flaxseed reverses protection of a reduced-fat diet against Citrobacter rodentium-induced colitis
Ground flaxseed reverses protection of a reduced-fat diet against Citrobacter rodentium-induced colitis
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DOI：
10.1152/ajpgi.00101.2018
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发表时间：
2018-11-01
影响因子：
4.5
通讯作者：
Sherman, Philip M.
中科院分区：
文献类型：
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作者：
Maattanen, Pekka;Lurz, Eberhard;Sherman, Philip M.
亚麻籽富含欧米茄-3多不饱和脂肪酸、纤维和木脂素，可以降低胆固醇水平。然而，它用于预防或治疗炎症性肠病产生了混合的结果，可能与饮食相互作用有关。本研究我们评估了在高脂（HF，类似于36%kcal）或低脂（RF，类似于12%kcal）饮食的情况下，补充磨碎的亚麻籽对柠檬酸杆菌诱导的结肠炎严重程度的影响。断奶后，C57 BL/6小鼠（n = 8-15/处理）在感染C.啮齿动物或假灌胃。在14天的感染期内，比较了体重变化、粘膜炎症、病原体负荷、肠道微生物群组成、组织多不饱和脂肪酸和盲肠短链脂肪酸。RF饮食保护了C。啮齿动物诱导的结肠炎，而RF Flx饮食增加了病原体负荷，加重了肠道炎症，并促进了肠道生态失调。当与RF饮食相比时，HF和HF Flx饮食均导致响应于C的更严重的病理。啮齿类感染我们的研究结果表明，虽然RF饮食可以保护C。新&值得注意的是，我们的研究结果表明，在柠檬酸杆菌引起的结肠炎期间，低脂饮食对肠道炎症、生态失调和病原体负担具有很强的保护作用。然而，地面亚麻籽补充在一个减少脂肪的饮食设置加剧结肠炎，尽管肠n-3多不饱和脂肪酸和盲肠短链脂肪酸的水平较高。
Flaxseed is high in omega-3 polyunsaturated fatty acids, fiber, and lignans known to lower cholesterol levels. However, its use for prevention or treatment of inflammatory bowel diseases has yielded mixed results, perhaps related to dietary interactions. In this study. we evaluated the impact of ground flaxseed supplementation on the severity of Citrobacter rodentium-induced colitis in the setting of either a high-fat (HF, similar to 36%kcal) or reduced-fat (RF, similar to 12%kcal) diet. After weaning, C57BL/6 mice (n = 8-15/treatment) were fed ground flaxseed (7 g/100 g diet) with either HF (HF Flx) or RF (RF Flx) diets for 4 wk before infection with C. rodentium or sham gavage. Weight changes, mucosal inflammation, pathogen burden, gut microbiota composition, tissue polyunsaturated fatty acids, and cecal short-chain fatty acids were compared over a 14-day infection period. The RF diet protected against C. rodentium-induced colitis, whereas the RF Flx diet increased pathogen burden, exacerbated gut inflammation, and promoted gut dysbiosis. When compared with the RF diet, both HF and HF Flx diets resulted in more severe pathology in response to C. rodentium infection. Our findings demonstrate that although an RF diet protected against C. rodentium-induced colitis and associated gut dysbiosis in mice, beneficial effects were diminished with ground flaxseed supplementation.NEW & NOTEWORTHY Our results demonstrate a strong protective effect of a reduced-fat diet against intestinal inflammation, dysbiosis, and pathogen burden during Citrobacter rodentium-induced colitis. However, ground flaxseed supplementation in the setting of a reduced-fat diet exacerbated colitis despite higher levels of intestinal n-3 polyunsaturated fatty acids and cecal short-chain fatty acids.