Flotillin-1 stabilizes caveolin-1 in intestinal epithelial cells.
Flotillin-1 stabilizes caveolin-1 in intestinal epithelial cells.
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DOI：
10.1016/j.bbrc.2008.12.118
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发表时间：
2009-02-06
影响因子：
3.1
通讯作者：
Nusrat, Asma
中科院分区：
文献类型：
--
作者：
Vassilieva, Elena V.;Ivanov, Andrei I.;Nusrat, Asma
Flotillins and caveolins represent two types of resident proteins associated with lipid rafts in mammalian cells, however, their possible cross-talk in regulating lipid raft functions remains poorly understood. In this report, we observed that siRNA-mediated down-regulation of flotillin-1 expression which disrupted lipid raft-mediated endocytosis of BODIPY FL C5-lactosylceramide also substantially decreased caveolin-1 level in SK-CO15 human intestinal epithelial cells. The decrease in caveolin-1 expression appeared to be specific for flotillin-1 knock-down and was not observed after down-regulation of flotillin-2. The decrease in caveolin-1 level in flotillin-1-depleted cells was not due to suppression of its mRNA synthesis and was not mimicked by cholesterol depletion of SK-CO15 cells. Furthermore, flotillin-1 dependent down-regulation of caveolin-1 was reversed after cell exposure to lysosomal inhibitor, chloroquine but not proteosomal inhibitor, MG262. Our data suggest that flotillin-1 regulates caveolin-1 level by preventing its lysosomal degradation in intestinal epithelial cells.
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