Enzymatic polymerization behavior using cellulose-binding domain deficient endoglucanase II.
Enzymatic polymerization behavior using cellulose-binding domain deficient endoglucanase II.
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使用纤维素结合域缺陷型内切葡聚糖酶 II 的酶促聚合行为。
DOI：
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发表时间：
2005
影响因子：
4.6
通讯作者：
S. Kimura
中科院分区：
文献类型：
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作者：
Itsuko Nakamura;H. Yoneda;T. Maeda;Akira Makino;M. Ohmae;J. Sugiyama;M. Ueda;Shiro Kobayashi;S. Kimura
一种突变酶，EGII（核心），其中纤维素结合结构域删除内切葡聚糖酶II从绿色木霉，在酵母中表达，分泌的酶进行了检查的酶促聚合，以获得人工纤维素。EGII（核）聚合的β-纤维二糖基氟化物，得到II型结晶纤维素。EGII（核）与EGII的聚合行为的比较揭示了以下内容：i）用EGII（核）获得的结晶产物在聚合溶液中是稳定的，尽管产物在EGII存在下容易水解; ii）EGII（核）的转换数与EGII一样高; iii）EGII（核）产生高度结晶的纤维素。因此，EGII（核心）对于酶促聚合是有利的。
A mutant enzyme, EGII(core), in which the cellulose-binding domain was deleted from endoglucanase II from Trichoderma viride, was expressed in yeast, and the secreted enzyme was examined for the enzymatic polymerization to obtain artificial cellulose. EGII(core) polymerized beta-cellobiosyl fluoride to afford crystalline cellulose of type II. Comparison of the polymerization behavior of EGII(core) with that of EGII revealed the following: i) the crystalline product obtained with EGII(core) was stable in the polymerization solution, although the product was readily hydrolyzed in the presence of EGII; ii) the turnover number of EGII(core) was as high as that of EGII; iii) EGII(core) produced highly crystalline cellulose. EGII(core) is therefore advantageous for enzymatic polymerization.