Correlation of 4'-[methyl-11C]-thiothymidine uptake with Ki-67 immunohistochemistry and tumor grade in patients with newly diagnosed gliomas in comparison with 11C-methionine uptake
Correlation of 4'-[methyl-11C]-thiothymidine uptake with Ki-67 immunohistochemistry and tumor grade in patients with newly diagnosed gliomas in comparison with 11C-methionine uptake
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DOI：
10.1007/s12149-015-1035-x
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发表时间：
2016-02-01
影响因子：
2.6
通讯作者：
Nishiyama, Yoshihiro
中科院分区：
文献类型：
--
作者：
Tanaka, Kenichi;Yamamoto, Yuka;Nishiyama, Yoshihiro
基于DNA掺入法，开发了一种新的放射性药物4 '-[甲基-C-11]硫代胸苷（C-11-4DST）作为体内细胞增殖标记物。为探讨C-11 - 4DST与l-[甲基-C-11]-蛋氨酸（C-11-MET）在新诊断胶质瘤患者中的摄取情况，并与肿瘤增殖活性和肿瘤分级进行比较，对23例新诊断胶质瘤患者进行了回顾性C-11-4DST和C-11-MET PET/CT检查。计算肿瘤的最大标准摄取值（SUVmax）（T）和正常对侧半球的平均SUV（N），并确定肿瘤与正常（T/N）的比值。代谢肿瘤体积（MTV）定义为阈值为SUVmax 40%的体积。在23例胶质瘤中，C-11-4DST PET/CT和C-11-MET PET/CT分别检测到20例和22例。C-11-4DST和C-11-MET之间的线性回归分析显示SUVmax（r = 0.54，P < 0.008）、T/N比值（r = 0.56，P < 0.006）和MTV（r = 0.60，P < 0.003）弱相关。直线回归分析显示C-11-4DST与Ki-67指数的SUVmax（r = 0.46，P < 0.03）、T/N比值（r = 0.43，P < 0.05）和MTV（r = 0.68，P < 0.001）以及C-11-MET MTV与Ki-67指数（r = 0.43，P < 0.04）之间存在弱相关性。C-11-4DST显示，Ⅱ级与Ⅳ级之间SUVmax差异有统计学意义（P < 0.03），Ⅱ级与Ⅳ级之间MTV差异有统计学意义（P < 0.009），Ⅲ级与Ⅳ级之间MTV差异有统计学意义（P < 0.02）。C-11-MET PET/CT的SUVmax（P < 0.009）、T/N（P < 0.02）、MTV（P < 0.03）在II级和IV级之间差异有统计学意义（P <0.02），C-11-4DST PET/CT和C-11-MET PET/CT同样适用于脑胶质瘤的显像。尤其是在新诊断胶质瘤中，C-11-4DST MTV与Ki-67指数的相关性最高。
A novel radiopharmaceutical, 4'-[methyl-C-11]thiothymidine (C-11-4DST), has been developed as an in vivo cell proliferation marker based on the DNA incorporation method. The purpose of this study was to evaluate C-11-4DST uptake in patients with newly diagnosed glioma and to correlate the results with proliferative activity and tumor grade, in comparison with l-[methyl-C-11]-methionine (C-11-MET).Investigations of C-11-4DST and C-11-MET PET/CT were performed retrospectively in 23 patients with newly diagnosed glioma. The maximum standardized uptake value (SUVmax) for tumor (T) and the mean SUV for normal contralateral hemisphere (N) were calculated, and the tumor-to-normal (T/N) ratio was determined. Metabolic tumor volume (MTV) was defined as the volume with a threshold of 40 % of the SUVmax. Proliferative activity as indicated by the Ki-67 index was estimated in tissue specimens.Of 23 gliomas examined, C-11-4DST PET/CT and C-11-MET PET/CT detected 20 and 22, respectively. Linear regression analysis between C-11-4DST and C-11-MET indicated a weak correlation for SUVmax (r = 0.54, P < 0.008), for T/N ratio (r = 0.56, P < 0.006), and for MTV (r = 0.60, P < 0.003). Linear regression analysis indicated a weak correlation between C-11-4DST and Ki-67 index for SUVmax (r = 0.46, P < 0.03), for T/N ratio (r = 0.43, P < 0.05), and for MTV (r = 0.68, P < 0.001) and between C-11-MET MTV and Ki-67 index (r = 0.43, P < 0.04). Using C-11-4DST, there was a significant difference in SUVmax between grades II and IV (P < 0.03) and in MTV between grades II and IV (P < 0.009) and grades III and IV (P < 0.02). Using C-11-MET, there was a significant difference in SUVmax (P < 0.009) and T/N ratio (P < 0.02) between grades II and IV and in MTV between grades II and IV (P < 0.03) and grades III and IV (P < 0.02).C-11-4DST PET/CT is feasible for imaging of brain gliomas, as well as C-11-MET PET/CT. Especially, it showed the highest correlation coefficient between C-11-4DST MTV and Ki-67 index in newly diagnosed gliomas.