Vascular tissue-specific expression of BnaC4.BOR1;1c, an efflux boron transporter gene, is regulated in response to boron availability for efficient boron acquisition and distribution in Brassica napus
Vascular tissue-specific expression of BnaC4.BOR1;1c, an efflux boron transporter gene, is regulated in response to boron availability for efficient boron acquisition and distribution in Brassica napus
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BnaC4.BOR1;1c（一种外排硼转运蛋白基因）的血管组织特异性表达根据硼的可用性进行调节，以实现甘蓝型油菜中硼的有效获取和分配
DOI：
10.1007/s11104-021-04985-y
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发表时间：
2021
期刊：
影响因子：
4.9
通讯作者：
Fangsen Xu
中科院分区：
文献类型：
--
作者：
Sheliang Wang;Ling Liu;Dan Zou;Yupu Huang;Zhe Zhao;Guangda Ding;Hongmei Cai;Chuang Wang;Lei Shi;Fangsen Xu
BnaC4.BOR1;1cis是甘蓝型油菜（Brassica napus，B. napus）在低B胁迫下的生长。本研究旨在揭示BnaC 4. BOR 1; 1c的B调控机制及其在B从根向地上部转运和B在地上部分布中的生理作用。原位PCR和免疫组化检测B.以油菜为材料研究BnaC4.BOR1; 1c的表达模式和定位。此外，还测定了野生型B.甘蓝型油菜和BnaC4.BOR1;结果BnaC 4. BOR 1;1cmRNA丰度与B利用率呈负相关，这种负相关是由5 '-UTR 5′端29 nt序列介导的。此外，5 '-UTR同时调节蛋白质表达水平，最可能取决于翻译效率。BnaC4.BOR1; 1c主要定位于根和茎中维管束细胞的质膜上，极性定位方式可能有利于B在根中木质部的装载和B在茎中木质部向韧皮部的卸载。短期10 B吸收分析表明，BnaC 4. BOR 1; 1c在B缺乏时优先将B分配给发育中的叶片和花。结论该研究揭示了BnaC 4. BOR 1; 1c中5 '-UTR介导的mRNA丰度和翻译效率对B可用性的联合调节作用及其在B发育组织优先获得B中的生理作用。油菜。
AimsBnaC4.BOR1;1cis required for B acquisition inBrassica napus(B. napus) under low B stress. This study aimed to reveal the B regulatory mechanism ofBnaC4.BOR1;1cand its physiological roles in B translocation from roots to shoots and B distribution in shoots.MethodsTransgenicArabidopsisplants expressing GUS (β-glucuronidase) under different promoters were generated and the mRNA, and GUS activity was quantitatively measured. The in-situ PCR and immunohistochemistry inB. napuswere performed to investigateBnaC4.BOR1;1cexpression pattern and localization. Furthermore, assays of B transport and distribution in wild typeB. napusandBnaC4.BOR1;1cRNAi lines were carried out to elucidate its physiological roles.ResultsResults showed thatBnaC4.BOR1;1cmRNA abundance is negatively correlated with B availability, which was mediated by the 29 nt sequence in the 5′ terminal region of 5’-UTR. Besides, the 5’-UTR simultaneously regulates protein expression level, most probably depending on the translation efficiency. BnaC4.BOR1;1c mainly localizes on the plasma membrane of vascular bundle cells in roots and shoots with a polar localization manner that is probably beneficial to B xylem loading in roots and B unloading from xylem to phloem in vascular bundle of shoots. Short-term10B uptake analysis demonstrates thatBnaC4.BOR1;1cpreferentially distributes B to developing leaves and flowers under B deficiency.ConclusionThis study reveals combined regulatory action of mRNA abundance and translation efficiency mediated by the 5’-UTR inBnaC4.BOR1;1cin response to B availability and its physiological role in preferential B acquisition in developing tissues ofB. napus.