Transcriptomic Profiling Reveals Complex Molecular Regulation in Cotton Genic Male Sterile Mutant Yu98-8A.
Transcriptomic Profiling Reveals Complex Molecular Regulation in Cotton Genic Male Sterile Mutant Yu98-8A.
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转录组分析揭示棉花雄性不育突变体 Yu98-8A 中复杂的分子调控
DOI：
10.1371/journal.pone.0133425
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Yang X
中科院分区：
文献类型：
--
作者：
Fang W;Zhao F;Sun Y;Xie D;Sun L;Xu Z;Zhu W;Yang L;Zhao Y;Lv S;Tang Z;Nie L;Li W;Hou J;Duan Z;Yu Y;Yang X
棉花核雄性不育在杂种优势利用中起着重要作用，但其确切的分子机制尚不清楚。为了研究棉花不育系育98 -8A花粉败育的分子机制，本研究结合组织学观察，对育98 -8A的花粉败育进行了转录组分析。在花粉败育的关键时期之前和期间，共有2，412个基因被鉴定为显著差异表达基因（DEG）。生物信息学和生物化学分析表明，主要与糖和淀粉代谢、氧化磷酸化和植物内源激素有关的DEG在花粉败育中起着关键而复杂的作用。这些结果有助于深入了解棉花核不育花粉败育的分子调控机制，为棉花杂种优势育种的进一步研究奠定基础。
Although cotton genic male sterility (GMS) plays an important role in the utilization of hybrid vigor, its precise molecular mechanism remains unclear. To characterize the molecular events of pollen abortion, transcriptome analysis, combined with histological observations, was conducted in the cotton GMS line, Yu98-8A. A total of 2,412 genes were identified as significant differentially expressed genes (DEGs) before and during the critical pollen abortion stages. Bioinformatics and biochemical analysis showed that the DEGs mainly associated with sugars and starch metabolism, oxidative phosphorylation, and plant endogenous hormones play a critical and complicated role in pollen abortion. These findings extend a better understanding of the molecular events involved in the regulation of pollen abortion in genic male sterile cotton, which may provide a foundation for further research studies on cotton heterosis breeding.