Phenotype character of the methylglyoxal resistance gene in Saccharomyces cerevisiae: expression in Escherichia coli and application to breeding wild-type yeast strains
Phenotype character of the methylglyoxal resistance gene in Saccharomyces cerevisiae: expression in Escherichia coli and application to breeding wild-type yeast strains
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酿酒酵母甲基乙二醛抗性基因的表型特征：在大肠杆菌中的表达及其在野生型酵母菌株选育中的应用
DOI：
10.1128/aem.50.5.1200-1207.1985
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发表时间：
1985
影响因子：
4.4
通讯作者：
A. Kimura
中科院分区：
文献类型：
--
作者：
K. Murata;Y. Fukuda;M. Shimosaka;K. Watanabe;T. Saikusa;A. Kimura
克隆了酿酒酵母抗丙酮醛基因，并对其表型特征进行了研究。S.具有该基因的酿酒酵母细胞可以在培养基中积累大量的谷胱甘肽，并且对各种毒性化合物如甲基乙二醛、二硫化四甲基秋兰姆、碘乙酰胺和重金属离子具有显著的高抗性。该基因在大肠杆菌中也得到了表达，并对大肠杆菌的抗性进行了初步研究。大肠杆菌细胞对有毒化合物的毒性也增加，如S.酿酒酵母细胞该基因的表型特征适用于无遗传标记的野生型酵母菌株转化子的选择。
The gene responsible for the methylglyoxal resistance of Saccharomyces cerevisiae was cloned, and its phenotypic characteristics were investigated. S. cerevisiae cells with the gene could accumulate large amounts of glutathione in the medium and should remarkably high resistance to various toxic compounds such as methylglyoxal, tetramethylthiuram disulfide, iodoacetamide, and heavy-metal ions. The gene was also expressed in Escherichia coli cells, and the resistance of E. coli cells to toxic compounds also increased as observed for S. cerevisiae cells. The phenotypic characteristics of the gene were applicable to the selection of the transformants of wild-type yeast strains having no genetic markers.