Development of Noninvasive In Vivo Approach to Assess Vascular Permeability in Inflammation Using Fluorescence Imaging.
Development of Noninvasive In Vivo Approach to Assess Vascular Permeability in Inflammation Using Fluorescence Imaging.
复制标题
使用荧光成像评估炎症血管通透性的无创体内方法的发展。
DOI：
10.1097/shk.0000000000001075
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
Oda S.
中科院分区：
文献类型：
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作者：
Shimazui T;Nakada TA;Fujimura L;Sakamoto A;Hatano M;Oda S.
方法：伊文思蓝(EB)与GENHANCE 750荧光染料混合注射小鼠。在盲肠结扎和穿孔(CLP)诱导的全身性炎症小鼠模型中，评价了作为远处器官的肺和作为非侵入性观察部位的足垫，并与假手术和氢化可的松预处理(CLP+HC)小鼠模型进行了比较。收集的组织中EB的提取被评估为血管通透性的常规指标。结果：CLP组足垫和肺组织的荧光强度显著高于其他两组(Foot Pad，Sham vs CLP，P<；0.0001；CLP vs CLP+HC，P=0.0004；Sham vs CLP+HC，P=0.058；肺，Sham vs CLP，P<；0.0001；CLP vs CLP+HC，P<；0.0001；Sham vs CLP+HC，P=0.060)。足垫的荧光强度与肺的荧光强度呈强相关(r=0.95)。结论：该荧光技术可用于基于EB定量的血管通透性评价。足垫荧光强度与肺内荧光强度高度相关，可作为无创性评价肺血管通透性的指标。
Methods:Evans blue (EB) and Genhance 750 fluorescent dye were mixed and injected into mice. The lung as a remote organ and the footpad as a noninvasive observational site were assessed in a cecal ligation and puncture (CLP)-induced systemic inflammation mouse model and compared with sham and hydrocortisone pretreated (CLP+ HC) mouse models. Extraction of EB in harvested tissues was assessed as a conventional indicator of vascular permeability. Fluorescent intensities in the footpad or harvested lung were assessed and their correlation was analyzed to investigate this novel, noninvasive approach for estimation of lung vascular permeability.Results:Fluorescent intensity in the footpad and harvested lung in the CLP group was significantly higher than in the other groups (footpad, sham vs. CLP, P< 0.0001; CLP vs. CLP+ HC, P= 0.0004; sham vs. CLP+ HC, P= 0.058; lung, sham vs. CLP, P< 0.0001; CLP vs. CLP+ HC, P< 0.0001; sham vs. CLP+ HC, P= 0.060). The fluorescent intensity in the footpad was strongly correlated with that in the lung (r= 0.95).Conclusions:This fluorescent technique may be useful for vascular permeability assessment based on EB quantification. Footpad fluorescent intensity was strongly correlated with that in the lung, and may be a suitable indicator in noninvasive estimation of lung vascular permeability.