Development and characterization of a rabbit alveolar bone nonhealing defect model.
Development and characterization of a rabbit alveolar bone nonhealing defect model.
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DOI：
10.1002/jbm.a.31639
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发表时间：
2008-07
期刊：
影响因子：
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通讯作者：
S. Young;Alex G. Bashoura;T. Borden;L. Baggett;J. Jansen;M. Wong;A. Mikos
中科院分区：
文献类型：
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作者：
S. Young;Alex G. Bashoura;T. Borden;L. Baggett;J. Jansen;M. Wong;A. Mikos
本研究的目的是在兔下颌骨中建立一种易于获得和可重复的、不可愈合的牙槽骨缺损区。成年雄性新西兰大白兔24只，行单侧下颌骨缺损手术。对前磨牙/磨牙区的两种类型的缺损进行了比较：(1)10 mm的“全厚”圆柱形缺损切除了皮质板和中间的小梁骨和牙根；(2)10 mm的“部分厚度”圆柱形缺损只切除了外侧骨皮质、小梁骨和牙根。在0、8和16周时，使用组织学和/或微型计算机断层扫描检查这两种类型的缺损区，以确定骨形成的质量和数量。8周时部分厚度缺损区可见明显的骨填充(86.9%+/-10.8%)，16周时骨轮廓完全再生并桥接。相反，全层缺损区永远不能桥接自身，在8周(61.5%+/-3.7%)和16周(55.1%+/-18.5%)时间点的骨再生方面没有显著差异。这些结果表明，在兔下颌前磨牙/磨牙区放置一个10 mm的双皮质圆柱形截骨可以造成无法愈合的缺陷，表明该动物模型作为下颌生物材料和组织工程构建的试验台的潜力。
The aim of this study was to develop an easily accessible and reproducible, nonhealing alveolar bone defect in the rabbit mandible. Twenty-four adult male New Zealand white rabbits underwent unilateral mandibular defect surgery. Two types of defect in the premolar/molar region were compared: (1) a 10-mm "full thickness" cylindrical defect removing both cortical plates and the intervening trabecular bone and tooth roots; (2) a 10-mm "partial thickness" cylindrical defect removing only the lateral bony cortex, trabecular bone, and tooth roots. Both types of defect were examined at 0, 8, and 16 weeks using histology and/or microcomputed tomography to determine the quality and quantity of bone formation. The partial thickness defect displayed significant bone fill at 8 weeks (86.9% +/- 10.8%), and complete regeneration of bony contours and bridging by 16 weeks. In contrast, the full thickness defect was never able to bridge itself and displayed no significant difference in bone regeneration between the 8-week (61.5% +/- 3.7%) and 16-week (55.1% +/- 18.5%) time points. These results indicate that a nonhealing defect can be created with a 10-mm bicortical cylindrical ostectomy placed in the premolar/molar region of the rabbit mandible, demonstrating the potential of this animal model as a test bed for mandibular biomaterials and tissue-engineering constructs.