Interferon messenger RNA is produced constitutively in the organs of normal individuals.
Interferon messenger RNA is produced constitutively in the organs of normal individuals.
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干扰素信使RNA在正常个体的器官中组成型产生。
DOI：
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发表时间：
1987
影响因子：
11.1
通讯作者：
M. Gigou
中科院分区：
文献类型：
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作者：
M. Tovey;M. Streuli;I. Gresser;J. Gugenheim;B. Blanchard;J. Guymarho;F. Vignaux;M. Gigou
用高比活性的DNA或RNA探针进行RNA印迹杂交，表明干扰素（IFN）-α mRNA组成型存在于正常个体的脾、肾、肝和外周血白细胞中。在从人体器官分离的poly（A）+ RNA中检测到单一条带（约等于1.2个酶）。该RNA与人IFN-α 1 DNA特异性杂交，并与来自病毒诱导的人外周血白细胞的成熟IFN-α mRNA共迁移。在任何测试的组织中均未检测到IFN-β RNA转录物。仅在一个正常人脾脏样本中检测到IFN-γ mRNA，该样本也含有异常高水平的IFN-α mRNA。使用修改的S1映射技术揭示了IFN-α 1和α 2转录本的存在。在同一样本中未检测到IFN-α 4、IFN-α 5、IFN-α 6、IFN-α 7、IFN-α 8或IFN-α 14转录物。在所有检测的样品中，IFN基因表达的特征模式的检测，不同于诱导后获得的模式，连同存在的低数量的转录物（小于或等于0.03拷贝/细胞）表明特异性IFN基因在体内组成型转录。
The use of RNA blot hybridization with DNA or RNA probes of high specific activity has shown that interferon (IFN)-alpha mRNA is present constitutively in the spleen, kidney, liver, and peripheral blood leukocytes of normal individuals. A single band (approximately equal to 1.2 kilobases) was detected in poly(A)+ RNA isolated from human organs. This RNA hybridized specifically to human IFN-alpha 1 DNA and comigrated with mature IFN-alpha mRNA from virus-induced human peripheral blood leukocytes. No IFN-beta RNA transcripts were detected in any of the tissues tested. IFN-gamma mRNA was detected in only one sample of normal human spleen, which also contained an unusually high level of IFN-alpha mRNA. The use of a modified S1 mapping technique revealed the presence of IFN-alpha 1 and -alpha 2 transcripts only. No IFN-alpha 4, -alpha 5, -alpha 6, -alpha 7, -alpha 8, or -alpha 14 transcripts were detected in the same sample. The detection, in all the samples tested, of a characteristic pattern of expression of IFN genes, different from that obtained following induction, together with the low number of transcripts present (less than or equal to 0.03 copy per cell) suggest that specific IFN genes are transcribed constitutively in vivo.