Isolation of neural stem cells from the postnatal cerebellum
Isolation of neural stem cells from the postnatal cerebellum
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DOI：
10.1038/nn1473
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发表时间：
2005-06-01
影响因子：
25
通讯作者：
Wechsler-Reya, RJ
中科院分区：
文献类型：
--
作者：
Lee, A;Kessler, JD;Wechsler-Reya, RJ
小脑对运动协调和认知功能至关重要，是髓母细胞瘤转化的目标，髓母细胞瘤是儿童最常见的恶性脑肿瘤。尽管对小脑中最丰富的神经元颗粒细胞的发育进行了详细的研究，但对其他小脑神经元和胶质细胞的起源仍然知之甚少。在这里，我们发现小鼠出生后小脑含有多能神经干细胞（NSCs）。这些细胞可以通过表达NSC标记物-1 （CD133）和缺乏神经元和胶质谱系标记物（lin(-)）进行前瞻性分离。纯化的突起（+）lin（-）细胞可形成自我更新的神经球，并在体外分化为星形胶质细胞、少突胶质细胞和神经元。此外，它们可以在移植到小脑后产生这些谱系。小脑干细胞的鉴定对理解小脑发育和成神经管细胞瘤的起源具有重要意义。
The cerebellum is critical for motor coordination and cognitive function and is the target of transformation in medulloblastoma, the most common malignant brain tumor in children. Although the development of granule cells, the most abundant neurons in the cerebellum, has been studied in detail, the origins of other cerebellar neurons and glia remain poorly understood. Here we show that the murine postnatal cerebellum contains multipotent neural stem cells (NSCs). These cells can be prospectively isolated based on their expression of the NSC marker prominin-1 (CD133) and their lack of markers of neuronal and glial lineages (lin(-)). Purified prominin(+)lin(-) cells form self-renewing neurospheres and can differentiate into astrocytes, oligodendrocytes and neurons in vitro. Moreover, they can generate each of these lineages after transplantation into the cerebellum. Identification of cerebellar stem cells has important implications for the understanding of cerebellar development and the origins of medulloblastoma.