Field effect detection of biomolecular interactions
Field effect detection of biomolecular interactions
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DOI：
10.1016/j.electacta.2005.02.075
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发表时间：
2005-09-05
影响因子：
6.6
通讯作者：
Migliorato, P
中科院分区：
文献类型：
--
作者：
Estrela, P;Stewart, AG;Migliorato, P
我们报告了使用场效应器件（例如金属氧化物半导体（MOS）电容器结构和多晶硅薄膜晶体管（Poly-Si TFT））检测生物分子相互作用。我们开发了带有氮化硅传感层的离子敏感场效应晶体管。已达到接近理想的 pH 灵敏度。通过用青霉素酶对传感区域进行功能化来制造青霉素传感器。使用 MOS 电容器和多晶硅 TFT 已经实现了 DNA 杂交的无标记电检测。器件金属栅极处发生的 DNA 相互作用导致栅极上的负电荷增加。电荷的这种变化分别通过 TFT 或 MOS 二极管的电流-电压或电容-电压特性的显着变化来反映。该技术可以检测单碱基对 DNA 错配。该方法可以扩展到其他化学或生化系统，例如蛋白质和细胞。 (C) 2005 Elsevier Ltd. 保留所有权利。
We report the detection of biomolecular interactions using field effect devices such as metal-oxide-semiconductor (MOS) capacitor structures and polycrystalline silicon thin film transistors (Poly-Si TFTs). We have developed ion-sensitive field effect transistors with a silicon nitride sensing layer. Near ideal pH sensitivity has been achieved. A penicillin sensor has been fabricated by functionalizing the sensing area with the enzyme penicillinase. Label-free electrical detection of DNA hybridization has been achieved using both MOS capacitors and Poly-Si TFTs. DNA interactions occurring at the metal gate of the devices lead to an increase of negative charges attached to the gate. This change in electrical charges is reflected by significant shifts of the current-voltage or capacitance-voltage characteristics of the TFTs or MOS diodes, respectively. Single base pair DNA mismatches can be detected with the technique. The method can be extended to other chemical or biochemical systems such as proteins and cells. (C) 2005 Elsevier Ltd. All rights reserved.