Age-related epileptogenic effects of corticotropin-releasing hormone in the isolated CA1 region of rat hippocampal slices.
Age-related epileptogenic effects of corticotropin-releasing hormone in the isolated CA1 region of rat hippocampal slices.
复制标题
促肾上腺皮质激素释放激素对大鼠海马切片 CA1 区的年龄相关致癫痫作用。
DOI：
10.1152/jn.1994.72.5.2328
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发表时间：
1994
影响因子：
2.5
通讯作者：
Dudek,FE
中科院分区：
文献类型：
--
作者：
Smith,BN;Dudek,FE
1. 研究促肾上腺皮质激素释放激素（CRH）对离体大鼠海马CA1区突触诱发的群体和细胞内反应的影响，以评价成年大鼠与幼年大鼠之间可能存在的差异。2. 0.2 ~ 0.6 μ m CRH可使幼年大鼠的正视诱发（辐射层刺激）种群峰值的幅度可逆性增强，且其程度大于成年大鼠。然而，在正常记录条件下，CRH没有引起癫痫样活动的发展。3. 在10-30微米双球茎碱的作用下，从出生后第8天开始，细胞内记录的动作电位和种群峰的间隙样爆发就可以被激发出来。CRH（0.2 ~ 0.6微米）可使幼年大鼠CA1区诱发的脉冲数量和持续时间可逆增加，且在更大程度上高于成年大鼠。4. 年轻大鼠和成年大鼠CA1锥体细胞胞内诱发动作电位爆发后的超极化幅度均降低0.2微米CRH，幅度相似。没有观察到输入电阻或膜电位的一致变化。5. 在对照组中，crh诱导的反应性增加幅度与初始兴奋性之间没有相关性，这表明crh诱导的变化与任何年龄相关的一般切片兴奋性差异无关。6. 我们的研究结果表明，在CA1区域，与成年大鼠相比，在年轻大鼠的切片中，CRH在更大程度上增强了双核碱诱导的爆发。（摘要删节250字）
1. The effects of corticotropin-releasing hormone (CRH) on synaptically evoked population and intracellular responses in the isolated rat CA1 region of hippocampal slices were studied to evaluate possible differences between adult and juvenile rats. 2. The amplitude of orthodromically evoked (stratum radiatum stimulation) population spikes was reversibly enhanced by 0.2–0.6 microM CRH to a greater extent in slices from juvenile rats than from adult rats. In no case, however, did CRH cause seizure-like activity to develop under normal recording conditions. 3. In the presence of 10–30 microM bicuculline, interictal-like bursts of population spikes and corresponding intracellularly recorded action potentials could be evoked starting at postnatal day 8. The number of spikes and the duration of the evoked bursts in the CA1 region were reversibly increased by CRH (0.2–0.6 microM) to a greater extent in slices from juvenile than from adult rats. 4. The amplitude of the afterhyperpolarization following intracellularly evoked bursts of action potentials in CA1 pyramidal cells was reduced by 0.2 microM CRH to a similar degree in both young and adult rats. No consistent changes in input resistance or membrane potential were observed. 5. No correlation was found between the magnitude of the CRH-induced increase in responsiveness and the initial excitability in controls, suggesting that the CRH-induced changes were independent of any age-dependent differences in general slice excitability. 6. Our results indicate that, in the CA1 region, CRH augments bicuculline-induced bursts to a greater extent in slices from young versus adult rats.(ABSTRACT TRUNCATED AT 250 WORDS)