THE DIFFERENTIATION OF NORMAL AND MUSCLE-FREE DISTAL CHICK LIMB BUD MESENCHYME IN MICROMASS CULTURE
THE DIFFERENTIATION OF NORMAL AND MUSCLE-FREE DISTAL CHICK LIMB BUD MESENCHYME IN MICROMASS CULTURE
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DOI：
10.1016/0012-1606(87)90216-8
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发表时间：
1987-01-01
影响因子：
2.7
通讯作者：
WOLPERT, L
中科院分区：
文献类型：
--
作者：
COTTRILL, CP;ARCHER, CW;WOLPERT, L
将第19至27期胚胎的鸡翅芽间充质在微团培养中生长。将包含位于顶端外胚层嵴350 μ m内的间充质的培养物（远端区间充质）的行为与紧邻近端间充质的培养物（近远端区培养物）的行为进行比较。在培养的远端间充质从阶段21-24肢体，所有的细胞染色免疫细胞化学II型胶原蛋白在3天内，表明无处不在的软骨分化。在第19和20阶段，仅在肢芽间充质的最远端50-100 μ m的培养物中观察到这种行为。在第25和27阶段之间，远端区培养物未能完全形成软骨。在所有阶段，近远侧区文化总是包含大量的非软骨细胞的区域。远端区和相应的subdistal区文化之间观察到的不同行为似乎是在后者体节衍生的假定的肌肉细胞的存在下的后果，因为没有这样的差异被观察到在类似的文化准备从无肌肉的翅芽。远端区的软骨分化的高能力支持其中抑制软骨是重要组成部分的模式形成的观点。然而，其在体外的一致行为表明，微团培养物不能反映不同阶段远端区之间的体内差异。近远侧区域保留了高的软骨分化能力，观察到的微团行为反映了与不同细胞群的相互作用。
Distal chick wing bud mesenchyme from stages 19 to 27 embryos has been grown in micromass culture. The behavior of cultures comprising mesenchyme located within 350 .mu.m of the apical ectodermal ridge (distal zone mesenchyme) was compared to that of cultures of the immediately proximal mesenchyme (subdistal zone cultures). In cultures of the distal mesenchyme from stages 21-24 limbs, all of the cells stained immunocytochemically for type II collagen within 3 days, indicating ubiquitous chondrogenic differentiation. At stage 19 and 20, this behavior was only observed in cultures of the distal most 50-100 .mu.m of the limb bud mesenchyme. Between stages 25 and 27, distal zone cultures failed to become entirely chondrogenic. At all stages, subdistal zone cultures always contained substantial areas of nonchondrogenic cells. The different behavior observed between distal zone and corresponding subdistal zone cultures appears to be a consequence of the presence of somite-derived presumptive muscle cells in the latter, since no such difference was observed in analogous cultures prepared from muscle-free wing buds. The high capacity of the distal zone for cartilage differentiation supports a view of pattern formation in which inhibition of cartilage is an important component. However, its consistent behavior in vitro indicates that micromass cultures do not reflect the in vivo differences between the distal zones at different stages. The subdistal region retains a high capacity of cartilage differentiation and the observed behavior in micromass reflects interactions with a different cell population.