Localization and expression of the alpha1A-1, alpha1B and alpha1D-adrenoceptors in hyperplastic and non-hyperplastic human prostate.
Localization and expression of the alpha1A-1, alpha1B and alpha1D-adrenoceptors in hyperplastic and non-hyperplastic human prostate.
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DOI：
10.1016/s0022-5347(01)61986-4
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发表时间：
1999-02
期刊：
影响因子：
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通讯作者：
P. Walden;Carl Gerardi;H. Lepor
中科院分区：
文献类型：
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作者：
P. Walden;Carl Gerardi;H. Lepor
目的研究α 1 A-1、α 1 D-肾上腺素受体（alpha 1 A-1，alpha 1 D-adrenoceptor，AR）亚型在增生和非增生前列腺组织中的表达和定位。在蛋白质水平上，采用亚型选择性抗体进行免疫组化。ARmRNA在增生组织和非增生组织中的表达差异无统计学意义，而α 1-AR亚型在两种组织中的表达比例差异有统计学意义。这些研究中最重要的发现是腺体和间质增生中alpha 1b-ARmRNA的表达减少。通过免疫组织化学，在间质中检测到α 1A-1-AR，而在腺上皮中未检测到。α 1B-AR主要位于上皮中，在间质中存在较弱。在增生的前列腺上皮中检测到较低水平的α 1B-AR。α 1D-AR在前列腺间质中表达，并大量存在于血管中。结论α 1A-1-、α 1B-和α 1D-AR在前列腺组织中的表达存在差异，且在BPH组织中表达水平均发生改变。BPH中α 1-AR亚型表达的改变（特别是α 1B-AR）不能仅仅归因于增生引起的组织形态学改变，并且可能在BPH的发病机制中具有重要意义。
PurposeTo determine the expression and localization of the alpha1A-1, alpha1Band alpha1D-adrenoceptor(AR) subtypes in hyperplastic and non-hyperplastic human prostate tissue.Materials and MethodsThe expression of the alpha1-ARsubtypes was examined at the mRNA level by quantitative solution hybridization, and at the protein level by immunohistochemistry using subtype selective antibodies.ResultsWhile the overall level of alpha1-ARmRNA was not significantly different between hyperplastic and non-hyperplastic tissue, there were significant differences in the ratio of the alpha1-ARsubtypes expressed in the two tissue types. The most significant finding from these studies was the reduced expression of the alpha1b-ARmRNA in both glandular and stromal hyperplasia. By immunohistochemistry, the alpha1A-1-ARwas detected in the stroma and not in the glandular epithelium. The alpha1B-ARwas localized predominantly in the epithelium and was weakly present in the stroma. Lower levels of the alpha1B-ARwere detected in the hyperplastic prostatic epithelium. The alpha1D-ARwas detected in areas of stroma and was abundantly present in blood vessels.ConclusionsThe alpha1A-1-, alpha1B-and alpha1D-ARsubtypes are differentially localized in human prostate, and the expression levels of all three subtypes are altered in BPH. Alterations in alpha1-ARsubtype expression (particularly the alpha1B-AR) in BPH cannot be solely attributed to changes in tissue morphometry resulting from hyperplasia and may be of significance in the pathogenesis of BPH.