THERMAL UNFOLDING OF YEAST GLYCINE TRANSFER-RNA
THERMAL UNFOLDING OF YEAST GLYCINE TRANSFER-RNA
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DOI：
10.1021/bi00654a013
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发表时间：
1976-01-01
期刊：
影响因子：
2.9
通讯作者：
RIESNER, D
中科院分区：
文献类型：
--
作者：
HILBERS, CW;ROBILLARD, GT;RIESNER, D
结合核磁共振、熔解技术和松弛动力学研究了酵母tRNAGly的分子去折叠。从这些研究中发现了以下解折叠途径：在偶联的解链转变中，三级、DHU [二氢尿苷]和反密码子结构被破坏。接着是受体臂的熔化，而仅包含Gcnt. C对的T ψ C臂最后熔化。有趣的是，在第一次熔融转变期间，形成了不属于原始三叶草结构的新结构。熔融转变的热力学和动力学参数的确定和讨论有关早期的工作。目前的NMR实验和早期的研究表明，环电流计算的基础上的苜蓿叶结构提供了一个很好的一级解释的NMR谱的tRNA。
The molecular unfolding of yeast tRNAGly was studied by a conbination of NMR spectroscopy, melting techniques and relaxation kinetics. From these studies the following pathway of unfolding was found. In a coupled melting transition the tertiary, the DHU [dihydrouridine] and the anticodon structure are disrupted. This is followed by the melting of the acceptor arm, while the T.psi.C arm, which only contains G.cntdot.C pairs, melts out last. Interestingly, during the first melting transition a new structure not belonging to the original cloverleaf structure is formed. The thermodynamic and kinetic parameters of the melting transitions were determined and are discussed in relation to earlier work. The present NMR experiments and earlier studies show that the ring current calculations based on the cloverleaf structure provide a good first-order interpretation of the NMR spectra of tRNA.