The 4E‐ms System of Producing Hybrid Wheat
The 4E‐ms System of Producing Hybrid Wheat
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杂交小麦生产的 4E-ms 系统
DOI：
10.2135/cropsci2005.0029
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发表时间：
2006
期刊：
影响因子：
2.3
通讯作者：
Genxuan Wang
中科院分区：
文献类型：
--
作者：
Kuanji Zhou;Shihong Wang;Yuqin Feng;Zhongxiang Liu;Genxuan Wang
提出了一种利用小麦雄性不育核基因和外源染色体获得授粉控制系统的杂种种子的细胞遗传学方法。本研究的目的是将外源染色体4 E导入单基因隐性雄性不育突变体兰州（LZ）中，建立一个有效的保持LZ雄性不育的细胞遗传学体系。以4 E二体附加系81529（2n = 44）为父本与LZ [2n = 42（msms）= 42]杂交，在F3代获得了淡蓝色籽粒的4 E单体附加系[2n = 43（msms）]。该品系为寄主小麦雄性不育基因纯合型，自交结实性好。自花受精种子分离为64.3%的白色（非蓝色）（2n = 42（msms））、32.1%的浅蓝色（2n = 43（msms））和3.6%的深蓝色（2n = 44（msms））。从白色谷粒生长的所有植物完全雄性不育。从浅蓝色籽粒生长的所有植株都具有良好的自交结实性，其后代种子在后代中再次分离为白色、浅蓝色和深蓝色籽粒。所有从深蓝色谷物生长的植物都是自花受精的，并在后代中保留了深蓝色。用白色不育系与任何品种作父本杂交，杂种植株均能恢复育性。这为建立小麦杂交种的4 E-ms体系奠定了基础。
A cytogenetic method for producing hybrid seed using a nuclear gene for male sterility and an alien chromosome to obtain a pollination control system has been proposed in bread wheat (Triticum aestivum L.). Our objective was to transfer the alien chromosome 4E to the monogenic recessive male-sterile mutant Lanzhou (LZ), and then to establish an efficient cytogenetic system of maintaining the male sterility of the LZ mutant. After crossing the 4E disomic addition line 81529 (2n = 44) as male to LZ [2n = 42(msms) = 42], we obtained the 4E monosomic addition line [2n = 43(msms)] in the F 3 generation that had light-blue seed color. The line was homozygous for the male-sterile gene (msms) of the host wheat and also had good self-fertility. The self-fertilized seeds segregated into 64.3% white (nonblue) with genotype 2n = 42(msms), 32.1% light blue with genotype 2n = 43(msms) and 3.6% deep blue with genotype 2n = 44(msms) grains. All plants grown from the white grains were completely male sterile. All plants grown from the light-blue grains had good self-fertility whose progeny seeds segregated into white, light-blue, and deep-blue grains again in succeeding generations. All plants grown from the deep-blue grains were self-fertile and retained their deep-blue color in the succeeding generations. When white grain male-sterile lines were crossed using any cultivar as the male parent, the hybrid plants had male fertility restored. And, this is the basis for the 4E-ms system of producing hybrid wheat.