Study of HLA-DR synthesis in cultured human keratinocytes.
Study of HLA-DR synthesis in cultured human keratinocytes.
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培养的人角质形成细胞中 HLA-DR 合成的研究。
DOI：
10.1111/1523-1747.ep12455746
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Weston,WL
中科院分区：
文献类型：
--
作者：
Wikner,NE;Huff,JC;Norris,DA;Boyce,ST;Cary,M;Kissinger,M;Weston,WL
在正常人表皮内，只有朗格汉斯细胞和不确定细胞表达 HLA-DR。然而，人角质形成细胞 (HK) 在某些以单核细胞浸润为特征的疾病状态下也可能表达 HLA-DR。先前的研究表明，HK 响应干扰素γ (INF-γ) 的刺激而合成 HLA-DR。本研究的目的是确定培养的 HK 可能表达 HLA-DR 的条件，并比较 HK 与单核细胞的 HLA-DR 合成。通过 HK 培养物的间接免疫荧光测定，HK 的 HLA-DR 表达在标准低钙条件下不存在，并且在添加钙、血清、丝裂原和含有表皮胸腺细胞激活因子的 Pam-212 细胞上清液后仍然不存在。 HR 中的 HLA-DR 表达是通过与刀豆球蛋白共培养来诱导的。 A 刺激外周血单核细胞 (PBMC)，但不是未刺激的 PBMC。这种效应是时间依赖性的，并且与 PBMC 的数量直接相关。通过添加来自刺激的PBMC (SS)的上清液或通过添加重组INF-γ但不通过添加白细胞介素(IL)-1或IL-2，还以时间和剂量依赖性方式诱导HLA-DR表达。 SS 或 INF-γ 的诱导被 INF-γ 抗血清阻断。通过半定量免疫沉淀技术测定，HK 的 HLA-DR 合成与 INF-γ 浓度直接相关。 HK产生的HLA-DR肽模式与单核细胞相似，但合成的相对量远少于单核细胞。
Within the normal human epidermis only Langerhans and indeterminate cells express HLA-DR. Human keratinocytes (HK), however, may also express HLA-DR in certain disease states characterized by mononuclear cell infiltrates. Previous studies have shown that HK synthesize HLA-DR in response to stimulation by interferon gamma (INF-γ). The purposes of this study were to define conditions under which cultured HK might express HLA-DR and to compare the HLA-DR synthesis of HK with that of monocytes. HLA-DR expression by HK as determined by indirect immunofluorescence of HK cultures was absent under standard low calcium conditions and remained absent with the addition of calcium, serum, mitogens, and supernatants from Pam-212 cells containing epidermal thymocyte- activating factor. HLA-DR expression in HR was induced by cocultivation with concanavalin. A-stimulated peripheral blood mononuclear cells (PBMC), but not unstimulated PBMC. This effect was time-dependent and directly related to the number of PBMC. HLA-DR expression was also induced in a time- and dose-dependent manner by addition of supernatant from stimulated PBMC (SS) or by addition of recombinant INF-γ but not by addition of interleukin (IL)-1 or IL-2. Induction by either SS or INF-γ was blocked by an antiserum to INF-γ. As determined by a semiquantitative immunoprecipitation technique, HLA-DR synthesis by HK was directly related to INF-γ concentration. The pattern of HLA-DR peptides produced by HK was similar to that of monocytes, but the relative quantity synthesized was far less than that of monocytes.