In Ganoderma lucidum, Glsnf1 regulates cellulose degradation by inhibiting GlCreA during the utilization of cellulose
In Ganoderma lucidum, Glsnf1 regulates cellulose degradation by inhibiting GlCreA during the utilization of cellulose
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DOI：
10.1111/1462-2920.14826
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发表时间：
2019-10-28
影响因子：
5.1
通讯作者：
Zhao, MingWen
中科院分区：
文献类型：
--
作者：
Hu, Yanru;Xu, Wenzhao;Zhao, MingWen
纤维素是农业生产的副产品，是一种丰富的废物。纤维素作为碳源，可被真菌降解利用。碳源作为营养物质，不仅提供能量，而且通过各种信号网络，使细胞能够感知和适应不同的环境条件，作为基因表达，代谢和生长的调节剂。营养传感途径优先使用优选的碳源，并在必要时调节纤维素降解酶的产生。了解真菌纤维素分解反应的调节将变得越来越重要，因为我们努力提高这些可再生能源的利用效率。在这里，我们表明，Glsnf 1，蔗糖非发酵丝氨酸-苏氨酸-蛋白激酶1（Snf 1）/AMP-活化的蛋白激酶同源物在药用大型担子菌灵芝，积极响应碳的变化，并积极调节纤维素酶活性和纤维素酶相关基因的转录。碳分解代谢物阻遏物CreA是一种锌双核簇转录因子，它介导营养物质的感知和抑制消耗较不优选的碳源所必需的许多基因的转录，参与Glsnf 1介导的纤维素酶调节。Glsnf 1不仅负调控CreA基因的转录水平，而且阻碍其在细胞核中的定位。总的来说，我们的研究结果揭示了一个关键的营养感应机制，这是至关重要的调制碳源适应在G。lucidum。
Cellulose is a by-product of agricultural production and an abundant waste. As a carbon source, cellulose can be degraded and utilized by fungi. Carbon sources, which act as nutrients, not only provide energy but also serve as regulators of gene expression, metabolism and growth, through various signalling networks that enable cells to sense and adapt to varying environmental conditions. Nutrient-sensing pathways prioritize the use of preferred carbon sources and regulate the production of cellulose-degrading enzymes when necessary. Understanding the regulation of the fungal cellulolytic response will become increasingly important because we strive to increase the efficiency of the utilization of these renewable energy sources. Here, we show that Glsnf1, a sucrose-nonfermenting serine-threonine-protein kinase 1 (Snf1)/AMP-activated protein kinase homologue in medicinal macro basidiomycete Ganoderma lucidum, actively responds to carbon alterations and positively regulates cellulase activity and cellulase-related gene transcription. The carbon catabolite repressor CreA, a zinc binuclear cluster transcription factor that mediates the sensing of nutrients and suppression of the transcription of a number of genes necessary for the consumption of a less preferred carbon source, participates in the Glsnf1-mediated regulation of cellulases. Glsnf1 not only negatively regulates the transcription level of the CreA gene but also hinders its localization in the nucleus. Overall, our findings reveal a key nutrient-sensing mechanism that is critical for the modulation of carbon source adaptation in G. lucidum.