Stimulation of GLUT-1 glucose transporter expression in response to hyperosmolarity
Stimulation of GLUT-1 glucose transporter expression in response to hyperosmolarity
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DOI：
10.1152/ajpcell.2001.281.4.c1365
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发表时间：
2001-10-01
影响因子：
5.5
通讯作者：
Ismail-Beigi, F
中科院分区：
文献类型：
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作者：
Hwang, DY;Ismail-Beigi, F
葡萄糖转运蛋白亚型-1（GLUT-1）的表达在应激条件下受到刺激。在这里，我们研究的机制介导的GLUT-1的表达增强的高渗。克隆9细胞暴露于600 mosmol/l（通过添加甘露醇产生）后，GLUT-1 mRNA、GLUT-1蛋白和葡萄糖转运增加。葡萄糖转运的刺激是双相的：在早期阶段（0-6小时），葡萄糖摄取的类似2.5倍刺激与GLUT-1 mRNA、GLUT-1蛋白或质膜中GLUT-1的含量无变化相关，而在晚期（12-24小时），葡萄糖转运的刺激增加了17倍，这与GLUT-1 mRNA的增加有关。（相似于7.5倍）和GLUT-1蛋白含量。暴露于高渗3小时后，细胞山梨醇增加。GLUT-1 mRNA含量的增加与mRNA半衰期从2小时增加到8小时相关。近端GLUT-1启动子中的44 bp区域是基础活性和高渗性表达增加2 - 3倍所必需的。可以得出结论，GLUT-1 mRNA含量的增加是由GLUT-1 mRNA的转录增强和稳定化介导的，并且与GLUT-1含量和葡萄糖转运活性的增加相关。
Glucose transporter isoform-1 (GLUT-1) expression is stimulated in response to stressful conditions. Here we examined the mechanisms mediating the enhanced expression of GLUT-1 by hyperosmolarity. GLUT-1 mRNA, GLUT-1 protein, and glucose transport increased after exposure of Clone 9 cells to 600 mosmol/l (produced by addition of mannitol). The stimulation of glucose transport was biphasic: in the early phase (0-6 h) a similar to2.5-fold stimulation of glucose uptake was associated with no change in the content of GLUT-1 mRNA, GLUT-1 protein, or GLUT-1 in the plasma membrane, whereas the similar to 17-fold stimulation of glucose transport during the late phase (12-24 h) was associated with increases in both GLUT-1 mRNA (similar to7.5-fold) and GLUT-1 protein content. Cell sorbitol increased after 3 h of exposure to hyperosmolarity. The increase in GLUT-1 mRNA content was associated with an increase in the half-life of the mRNA from 2 to 8 h. A 44-bp region in the proximal GLUT-1 promoter was necessary for basal activity and for the two- to threefold increases in expression by hyperosmolarity. It is concluded that the increase in GLUT-1 mRNA content is mediated by both enhanced transcription and stabilization of GLUT-1 mRNA and is associated with increases in GLUT-1 content and glucose transport activity.