Gβ1γ2 activates phospholipase A(2)-dependent Golgi membrane tubule formation.
Gβ1γ2 activates phospholipase A(2)-dependent Golgi membrane tubule formation.
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DOI：
10.3389/fcell.2014.00004
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发表时间：
2014-02-28
影响因子：
5.5
通讯作者：
Brown WJ
中科院分区：
文献类型：
--
作者：
Bechler ME;Brown WJ
异三聚体 G 蛋白将跨膜 G 蛋白偶联受体的配体结合转导至多种细胞内信号传导途径。最近，高尔基复合体的异三聚体 Gβγ 亚基信号传导已被证明可以调节囊泡运输载体的形成，从而将货物从高尔基体传递到质膜。除了囊泡外，膜小管也被证明可以介导高尔基复合体的输出，这需要细胞质磷脂酶 A2 (PLA2) 酶的活性。通过使用分离的高尔基复合体的体外重建测定，我们提供了 Gβ1γ2 信号传导也刺激高尔基膜小管形成的证据。此外，我们还发现 PLA2 酶的 Gβγ 激活抑制剂可抑制体外高尔基体膜小管的形成。此外，纯化的 Gβγ 蛋白在低（低于阈值）胞质浓度的情况下会刺激膜小管。重要的是，PLA2 拮抗剂 ONO-RS-082 治疗可抑制高尔基体膜小管形成的 Gβγ 刺激。这些研究表明，Gβ1γ2 信号传导激活高尔基体膜小管形成所需的 PLA2 酶，从而为该过程建立了新的调控层。
Heterotrimeric G proteins transduce the ligand binding of transmembrane G protein coupled receptors into a variety of intracellular signaling pathways. Recently, heterotrimeric Gβγ subunit signaling at the Golgi complex has been shown to regulate the formation of vesicular transport carriers that deliver cargo from the Golgi to the plasma membrane. In addition to vesicles, membrane tubules have also been shown to mediate export from the Golgi complex, which requires the activity of cytoplasmic phospholipase A2 (PLA2) enzyme activity. Through the use of an in vitro reconstitution assay with isolated Golgi complexes, we provide evidence that Gβ1γ2 signaling also stimulates Golgi membrane tubule formation. In addition, we show that an inhibitor of Gβγ activation of PLA2 enzymes inhibits in vitro Golgi membrane tubule formation. Additionally, purified Gβγ protein stimulates membrane tubules in the presence of low (sub-threshold) cytosol concentrations. Importantly, this Gβγ stimulation of Golgi membrane tubule formation was inhibited by treatment with the PLA2 antagonist ONO-RS-082. These studies indicate that Gβ1γ2 signaling activates PLA2 enzymes required for Golgi membrane tubule formation, thus establishing a new layer of regulation for this process.
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影响因子：
3.3
作者：
Puertollano, R;van der Wel, NN;Bonifacino, JS
通讯作者：
Bonifacino, JS
影响因子：
4.8
作者：
Irannejad, Roshanak;Wedegaertner, Philip B.
通讯作者：
Wedegaertner, Philip B.
影响因子：
5.8
作者：
HASHIZUME, T;YAMAGUCHI, H;FUJII, T
通讯作者：
FUJII, T
DOI：
10.1083/jcb.200612017
发表时间：
2007-07-02
期刊：
The Journal of cell biology
影响因子：
--
作者：
Fugmann T;Hausser A;Schöffler P;Schmid S;Pfizenmaier K;Olayioye MA
通讯作者：
Olayioye MA
影响因子：
64.5
作者：
Jamora, C;Takizawa, PA;Malhotra, V
通讯作者：
Malhotra, V