Development of probe electrospray using a solid needle
Development of probe electrospray using a solid needle
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DOI：
10.1002/rcm.3201
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发表时间：
2007-01-01
影响因子：
2
通讯作者：
Suzuki, Shigeo
中科院分区：
文献类型：
--
作者：
Hiraoka, Kenzo;Nishidate, Kentaro;Suzuki, Shigeo
发展了一种固体探针电喷雾分析液体样品的方法。针通过电机驱动系统沿垂直轴上下移动。在针的最低位置，针尖接触液体样品的表面，并且少量液体被针拾取。在最高位置处，将类似于3kV的高电压施加到针上，并且将装载在针尖上的样品电喷雾。通过正交飞行时间质谱仪测量形成的离子。通过针加载的单个样品给出了水溶液中的氨基酸、肽、蛋白质和聚乙二醇（PEG）的相当强的离子信号。向水溶液中加入盐或酸导致离子信号的显著增强。版权所有（c）2007约翰威利父子有限公司。
Electrospray using a solid needle probe for the analysis of liquid samples was developed. The needle was moved up and down a vertical axis by a motor-driven system. At the lowest position of the needle, the tip of the needle touched the surface of the liquid sample and a small amount of liquid was picked up by the needle. At the highest position, a high voltage of similar to 3 kV was applied to the needle and the sample loaded on the tip of the needle was electrosprayed. The ions formed were measured by an orthogonal time-of-flight mass spectrometer. A single sample loading by the needle gave reasonably strong ion signals for amino acids, peptides, proteins and polyethylene glycol (PEG) in aqueous solution. The addition of salts or acids to aqueous solution led to dramatic enhancement of the ion signals. Copyright (c) 2007 John Wiley & Sons, Ltd.