Dual-field and flow-programmed lift hyperlayer field-flow fractionation.
Dual-field and flow-programmed lift hyperlayer field-flow fractionation.
复制标题
双场和程序流升力超层场流分级。
DOI：
10.1021/ac00025a003
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发表时间：
1992
影响因子：
7.4
通讯作者：
Giddings,JC
中科院分区：
文献类型：
--
作者：
Ratanathanawongs,SK;Giddings,JC
FFF自成立以来的一项重要改进是编程。在程序FFF中，场强、流速或其他一些控制保留的参数在运行过程中是不同的，主要是为了加快缓慢迁移组分的洗脱速度。在FFF中编程的概念最早是由Yang、Myers和Gidding开发和应用的(10)。然而，这一初始编程概念以及随后报告的FFF的所有实验编程工作仅适用于所谓的FFF操作的正常模式(11-19)。这项工作的目的是证明编程不仅适用于FFF的另一种称为超层FFF的主要操作模式，而且对其高效，尤其是在称为Lift超层FFF的特定形式中，它是空间FFF的近亲。虽然粗略的分析表明，这种编程技术的扩展不会获得什么好处，但更彻底的分析为Lift超级层FFF的编程提供了明确的理由。文中给出了支持它的理论基础和实验证据。
One important improvement in FFF since its inception is that of programming. In programmed FFF, the field strength, the flow velocity, or some other retention-controlling param-eter is varied in the course of a run, primarily forthe purpose of speeding upthe elution of slowly migrating components. The concept of programming in FFF was first developed and applied by Yang, Myers, and Giddings (10). However, this initial programming concept, along with all subsequent ex-perimental programming work reported for FFF, has been applied only to the so-called normal mode of FFF operation (11-19). It is the object of this work to show that programming is not only applicable to, but highly effective with, another major operating mode of FFF termed hyperlayer FFF, par-ticularly in the specific form termed lift hyperlayer FFF, a close relative of steric FFF. While a cursory analysis suggests that little would be gained by such an extension of programming techniques, a more thorough analysis provides a clear rationale for the programming of lift hyperlayer FFF. The rationale and the experimental evidence in its support are presented in this paper.