CHD7 Regulates Osteogenic Differentiation of Human Dental Follicle Cells via PTH1R Signaling.
CHD7 Regulates Osteogenic Differentiation of Human Dental Follicle Cells via PTH1R Signaling.
复制标题
CHD7 通过 PTH1R 信号调节人牙囊细胞的成骨分化
DOI：
10.1155/2020/8882857
复制
发表时间：
2020
影响因子：
4.3
通讯作者：
Kang N
中科院分区：
文献类型：
--
作者：
Liu C;Li Q;Xiao Q;Gong P;Kang N
染色体结构域解旋酶DNA结合蛋白7（Chromodomain helicase DNA-binding protein 7，CHD 7）是一种ATP依赖的染色质重构酶，作为染色质阅读器进行表观遗传修饰。其对人牙囊细胞（hDFCs）成骨分化的影响尚不清楚。在这里，我们显示CHD 7表达随着成骨分化而增加。CHD 7的敲低损害了hDFC的成骨能力，其特征在于碱性磷酸酶活性和矿化降低，以及成骨相关基因的表达降低。相反，CHD 7过表达增强hDFC的成骨分化。从机制上讲，RNA-seq分析揭示了CHD 7敲低后PTH（甲状旁腺激素）/PTH 1 R（甲状旁腺激素受体-1）信号通路的下调富集。我们发现PTH 1 R的表达与CHD 7呈正相关。重要的是，PTH 1 R在CHD 7敲低的hDFC中的过表达部分地挽救了受损的成骨分化。我们的研究表明，CHD 7通过调节PTH 1 R的转录来调节hDFCs的成骨分化。
Chromodomain helicase DNA-binding protein 7 (CHD7) is an ATP-dependent chromatin remodeling enzyme, functioning as chromatin reader to conduct epigenetic modification. Its effect on osteogenic differentiation of human dental follicle cells (hDFCs) remains unclear. Here, we show the CHD7 expression increases with osteogenic differentiation. The knockdown of CHD7 impairs the osteogenic ability of hDFCs, characterized by reduced alkaline phosphatase activity and mineralization, and the decreased expression of osteogenesis-related genes. Conversely, the CHD7 overexpression enhances the osteogenic differentiation of hDFCs. Mechanically, RNA-seq analyses revealed the downregulated enrichment of PTH (parathyroid hormone)/PTH1R (parathyroid hormone receptor-1) signaling pathway after CHD7 knockdown. We found the expression of PTH1R positively correlates with CHD7. Importantly, the overexpression of PTH1R in CHD7-knockdown hDFCs partially rescued the impaired osteogenic differentiation. Our research demonstrates that CHD7 regulates the osteogenic differentiation of hDFCs by regulating the transcription of PTH1R.