Zebrafish elav/HuC homologue as a very early neuronal marker
Zebrafish elav/HuC homologue as a very early neuronal marker
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DOI：
10.1016/0304-3940(96)13021-4
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发表时间：
1996-09-27
影响因子：
2.5
通讯作者：
Miki, N
中科院分区：
文献类型：
--
作者：
Kim, CH;Ueshima, E;Miki, N
果蝇ELAV是一种神经元特异性RNA结合蛋白，在所有神经元出生后立即表达。这种特定的表达模式导致其用作泛神经元标记物。elav家族的至少三个成员HuD、HuC/ple 21和Hel-N1已被报道在脊椎动物中是神经元特异性的，尽管尚不清楚该家族的哪个成员在早期神经元确定时表达。我们已经分离到一个斑马鱼elav/HuC同源物（zHuC），它与人类HuC蛋白具有89%的同源性。它首先在原肠胚形成后立即在神经板中的神经元前体细胞中表达，然后在神经系统的大多数区域中持续高表达水平。HuC与果蝇中的elav一样，可能是斑马鱼最早的神经元标记物之一。
Drosophila ELAV, a neuron-specific RNA binding protein, is expressed in all neurons right after their birth. This specific pattern of expression has led to its use as a pan-neuronal marker. At least three members of the elav family, HuD, HuC/ple21 and Hel-N1, have been reported to be neuron-specific in vertebrates, although it is unknown which member of this family is expressed at the time of early neuronal determination. We have isolated a zebrafish elav/HuC homologue (zHuC) which has 89% homology to human HuC protein. It is first expressed in the neuronal precursor cells in the neural plate immediately after gastrulation, and then high expression levels persist in most regions of the nervous system. HuC, like elav in Drosophila, may be one of the earliest neuronal markers in zebrafish.