Distinct developmental expression of synaptotagmin I and IX in the mouse brain
Distinct developmental expression of synaptotagmin I and IX in the mouse brain
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DOI：
10.1097/01.wnr.0000198435.14142.be
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发表时间：
2006-02-06
期刊：
影响因子：
1.7
通讯作者：
Fukuda, M
中科院分区：
文献类型：
--
作者：
Fukuda, M
Synaptotagmin IX被认为是神经内分泌细胞中致密核心囊泡胞吐的主要Ca2+传感器。在这项研究中，我们研究了突触结合蛋白IX在小鼠脑中的亚细胞定位和发育表达谱，发现它主要存在于致密核心囊泡部分，这是缺乏突触结合蛋白I和突触素。我们还发现，突触结合蛋白IX的表达水平是恒定的，在整个出生后的小鼠大脑的发展，而突触结合蛋白I，II，III，VI和XII的上调与突触发生平行。这些发现表明，突触结合蛋白IX调节的transon。除了突触囊泡之外的脑内某些囊泡的结构。
Synaptotagmin IX has been postulated to function as a major Ca2+ sensor for dense-core vesicle exocytosis in neuroendocrine cells. In this study, we investigated the subcellular localization and developmental expression profile of synaptotagmin IX in the mouse brain and found that it is mainly present in the dense-core vesicle fraction, which is devoid of synaptotagmin I and synaptophysin. We also found that the synaptotagmin IX expression level is constant throughout the postnatal development of the mouse brain, whereas the synaptotagmins I, II, III, VI, and XII are upregulated in parallel with synaptogenesis. These findings suggest that synaptotagmin IX regulates the transpon. of certain vesicles in the brain other than synaptic vesicles.