AuNP aggregation-induced quantitative colorimetric aptasensing of sulfadimethoxine with a smartphone
AuNP aggregation-induced quantitative colorimetric aptasensing of sulfadimethoxine with a smartphone
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使用智能手机对磺胺二甲氧嘧啶进行 AuNP 聚集诱导的定量比色适体传感
DOI：
10.1016/j.cclet.2021.09.061
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发表时间：
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期刊：
影响因子：
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通讯作者：
Xiujun Li
中科院分区：
文献类型：
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作者：
Xiaoliang Zhang;Le Wang;Xiaochun Li;Xiujun Li
建立了一种智能手机上纳米金（AuNP）聚集诱导的比色适配传感方法，用于磺胺二甲氧嘧啶（SDM）的定量检测。金纳米粒子与适体复合，保护金纳米粒子在高浓度盐溶液中聚集。在SDM的存在下，SDM以更高的亲和力与金纳米粒子表面的适配体结合，竞争性地将适配体从金纳米粒子表面脱附，导致金纳米粒子聚集，并伴随颜色从红色变为紫蓝色。用智能手机上的应用程序分析由智能手机相机拍摄的图像的R、G和B值，并用于SDM的定量分析。在优化的条件下，使用智能手机的比色适配方法显示出对SDM的高灵敏度，检测限为0.023 ppm，低于允许的最大SDM残留限量。本研究为现场定量生化和细胞分析提供了一种简单、快速、易读的方法。
A gold nanoparticle (AuNP) aggregation-induced colorimetric aptasensing method for quantitative detection of sulfadimethoxine (SDM) with a smartphone was developed. AuNPs were complexed with aptamers which protected AuNPs from aggregating in high-concentration salt solutions. In the presence of SDM, SDM bound with the aptamer on the surface of AuNPs with higher affinity, which competitively desorbed the aptamer from the AuNP surface and resulted in AuNPs aggregation, accompanied with a color change from red to purple-blue. The R, G and B values of images taken by a smartphone camera were analyzed with an app on the smartphone, and were utilized for quantitative analysis of SDM. Under the optimized conditions, the colorimetric aptasensing method using a smartphone showed high sensitivity for SDM, with the limit of detection of 0.023 ppm, lower than the allowed maximum SDM residue limit. This study provides a simple, fast, and easy to read method for on-site quantitative biochemical and cellular analysis.