Attachment of anti-GFP antibodies to microspheres for optical trapping experiments.
Attachment of anti-GFP antibodies to microspheres for optical trapping experiments.
复制标题
将抗 GFP 抗体附着到微球上以进行光捕获实验。
DOI：
10.1101/pdb.prot066670
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发表时间：
2011
影响因子：
--
通讯作者：
Warrick,HansM
中科院分区：
文献类型：
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作者：
Spudich,JamesA;Rice,SarahE;Rock,RonaldS;Purcell,ThomasJ;Warrick,HansM
体外运动分析可以分析ATP水解与肌动蛋白沿肌动蛋白丝或肌动蛋白沿微管运动之间的耦合关系。使用激光捕获的单分子分析已被用于获得更多关于运动蛋白、肌凝蛋白和进程DNA酶的详细信息。体外运动测定与激光陷阱测量的结合揭示了与atp酶周期相关的详细动态结构变化。本方案描述了一种将抗gfp（绿色荧光蛋白）抗体附着到微球上的方法。gfp马达融合蛋白可以被吸附到微球上，用于单分子运动研究和光学捕获实验。
In vitro motility assays enabled the analysis of coupling between ATP hydrolysis and movement of myosin along actin filaments or kinesin along microtubules. Single-molecule assays using laser trapping have been used to obtain more detailed information about kinesins, myosins, and processive DNA enzymes. The combination of in vitro motility assays with laser-trap measurements has revealed detailed dynamic structural changes associated with the ATPase cycle. This protocol describes a method for attaching anti-GFP (green fluorescent protein) antibodies to microspheres. GFP-motor fusion proteins can then be adsorbed to the microspheres for use in single-molecule motility studies and optical trapping experiments.