A novel approach for myocardial regeneration with educated cord blood cells cocultured with cells from brown adipose tissue.
A novel approach for myocardial regeneration with educated cord blood cells cocultured with cells from brown adipose tissue.
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DOI：
10.1016/j.bbrc.2006.12.017
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发表时间：
2007-02
影响因子：
3.1
通讯作者：
Yoshihiro Yamada;S. Yokoyama;N. Fukuda;H. Kidoya;Xiaoyong Huang;Hisamichi Naitoh;Naoyuki Satoh;N. Takakura
中科院分区：
文献类型：
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作者：
Yoshihiro Yamada;S. Yokoyama;N. Fukuda;H. Kidoya;Xiaoyong Huang;Hisamichi Naitoh;Naoyuki Satoh;N. Takakura
脐带血（CB）是人类再生治疗的一个有前途的来源。最近，显示CB是间充质干细胞以及造血干细胞的来源，并且进一步显示间充质干细胞可以分化成间充质谱系的多种细胞类型，例如心肌细胞（CM）、骨细胞、软骨细胞和脂肪细胞。以前，我们报道了棕色脂肪组织来源的细胞（BATDC）分化为CM，这些CM可以在功能上适应心肌梗死区域的修复。在这项研究中，我们研究了CB单核细胞（CBMNCs）是否可以有效地分化为CM通过共培养他们与BATDC和确定CBMNCs中的群体分化为CM。结果表明，BATDC在体外有效地诱导CBMNC（非造血干细胞（HSC）（受教育的CB细胞：e-CBCs））成为CM。E-CBCs重建梗死心肌比未受过教育的CBMNC或CD 34阳性HSC更有效。此外，我们发现与BATDC共培养3天后的e-CBCs诱导受损CM最有效的再生。这表明e-CBC具有分化成CM的高潜力，并且适当的移植时机支持CM再生的高效率。这种策略可能是一种有前途的治疗人类心脏疾病的方法。
Umbilical cord blood (CB) is a promising source for regeneration therapy in humans. Recently, it was shown that CB was a source of mesenchymal stem cells as well as hematopoietic stem cells, and further that the mesenchymal stem cells could differentiate into a number of cells types of mesenchymal lineage, such as cardiomyocytes (CMs), osteocytes, chondrocytes, and fat cells. Previously, we reported that brown adipose tissue derived cells (BATDCs) differentiated into CMs and these CMs could adapt functionally to repair regions of myocardial infarction. In this study, we examined whether CB mononuclear cells (CBMNCs) could effectively differentiate into CMs by coculturing them with BATDCs and determined which population among CBMNCs differentiated into CMs. The results show that BATDCs effectively induced CBMNCs that were non-hematopoietic stem cells (HSCs) (educated CB cells: e-CBCs) into CMs in vitro. E-CBCs reconstituted infarcted myocardium more effectively than non-educated CBMNCs or CD34-positive HSCs. Moreover, we found that e-CBCs after 3 days coculturing with BATDCs induced the most effective regeneration for impaired CMs. This suggests that e-CBCs have a high potential to differentiate into CMs and that adequate timing of transplantation supports a high efficiency for CM regeneration. This strategy might be a promising therapy for human cardiac disease.