Rapid detection of porcine reproductive and respiratory syndrome virus by reverse transcription loop-mediated isothermal amplification assay
Rapid detection of porcine reproductive and respiratory syndrome virus by reverse transcription loop-mediated isothermal amplification assay
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DOI：
10.1016/j.jviromet.2008.09.012
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发表时间：
2009-01-01
影响因子：
3.1
通讯作者：
Cao, Yong-chang
中科院分区：
文献类型：
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作者：
Li, Qiang;Zhou, Qing-feng;Cao, Yong-chang
建立了一种基于逆转录环介导等温扩增（RT-LAMP）的猪繁殖与呼吸综合征病毒（PRRSV）快速检测方法。RT-LAMP试验利用一组六条引物扩增PRRSV的开放阅读框6（ORF 6）。扩增产物用琼脂糖凝胶电泳或比色法进行分析。结果表明，RT-LAMP检测检测了所有22种不同的PRRSV分离株，与其他四种猪病毒（即，PCV 2，SIV。CSFV和PEDV），并在23个阳性临床样品中获得了91.3%的灵敏度，参考允许的基于细胞的病毒分离程序。因此，RT-LAMP检测提供了一种特异性和敏感性的手段，用于检测PRRSV在一个简单的，快速的，和成本效益的方式。此外，RT-LAMP测定可以在装备不太好的实验室以及现场进行。(c)2008 Elsevier B. V.保留所有权利。
A rapid detection assay based on reverse transcription loop-mediated isothermal amplification (RT-LAMP) has been developed for detecting porcine reproductive and respiratory syndrome virus (PRRSV). The RT-LAMP assay utilized a set of six primers to amplify the open reading frame 6 (ORF6) of the PRRSV. The amplified products were analyzed by agarose gel electrophoresis or visualized by colorimetric method. The results demonstrated that the RT-LAMP assay detected all 22 different PRRSV isolates, had no cross-reaction with four other swine viruses (i.e., PCV2, SIV. CSFV, and PEDV), and obtained a 91.3% sensitivity in 23 positive clinical samples in reference to the permissive cells-based virus isolation procedure. Therefore, the RT-LAMP assay provides a specific and sensitive means for detecting PRRSV in a simple, fast, and cost-effective manner. Furthermore, the RT-LAMP assay can be performed in less well-equipped laboratories as well as fields. (c) 2008 Elsevier B.V. All rights reserved.