A multilocus genealogical approach to phylogenetic species recognition in the model eukaryote Neurospora
A multilocus genealogical approach to phylogenetic species recognition in the model eukaryote Neurospora
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DOI：
10.1111/j.0014-3820.2003.tb01514.x
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发表时间：
2003-12-01
期刊：
影响因子：
3.3
通讯作者：
Taylor, JW
中科院分区：
文献类型：
--
作者：
Dettman, JR;Jacobson, DJ;Taylor, JW
以系统发育和生殖相容性为标准，批判性地研究物种间的关系。我们将系统发育物种识别（PSR）应用于真菌，其中生物物种识别（BSR）已被最广泛地应用，即被充分研究的脉孢菌属（Neurospora）。四个独立的匿名核基因座的特点和测序147个人，代表所有描述的远交种脉孢菌。我们开发了一个共识树的方法，确定单系系谱组一致支持的大多数位点，或至少有一个位点支持，但不矛盾的任何其他位点。我们共识别出8个系统发育物种，其中5个与5个传统生物物种相对应，3个是新发现的。系统发育标准不仅上级优于传统的生殖相容性标准，揭示了完整的物种多样性的脉孢菌，但也显着的系统发育亚组内的一些物种。尽管以前的建议，杂交N。crassa和N.尽管本研究中包括了几个假定的杂种个体，但没有观察到支持最近种间基因流动或真正杂种存在的分子证据。将这四个位点的序列数据结合起来，用于阐明PSR发现的物种之间的关系。虽然物种水平的分支得到了有力的支持，物种之间的系统发育关系仍然难以解决，可能是由于冲突的信号所造成的差异谱系排序。
To critically examine the relationship between species recognized by phylogenetic and reproductive compatibility criteria. we applied phylogenetic species recognition (PSR) to the fungus in which biological species recognition (BSR) has been most comprehensively applied, the well-studied genus Neurospora. Four independent anonymous nuclear loci were characterized and sequenced from 147 individuals that were representative of all described outbreeding species of Neurospora. We developed a consensus-tree approach that identified monophyletic genealogical groups that were concordantly supported by the majority of the loci, or were well supported by at least one locus but not contradicted by any other locus. We recognized a total of eight phylogenetic species, five of which corresponded with the five traditional biological species, and three of which were newly discovered. Not only were phylogenetic criteria superior to traditional reproductive compatibility criteria in revealing the full species diversity of Neurospora, but also significant phylogenetic subdivisions were detected within some species. Despite previous suggestions of hybridization between N. crassa and N. intermedia in nature, and the fact that several putative hybrid individuals were included in this study, no molecular evidence in support of recent interspecific gene flow or the existence of true hybrids was observed. The sequence data from the four loci were combined and used to clarify how the species discovered by PSR were related. Although species-level clades were strongly supported, the phylogenetic relationships among species remained difficult to resolve, perhaps due to conflicting signals resulting from differential lineage sorting.